Biochemical/Cell-based Assays, Reagents & HTS Kits

For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate for 20 minute incubation at 25°C: 0.02 to 10 U/L glucose oxidase for colorimetric assays and 0.002 to 1.5 U/L for fluorimetric assays. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, FL530/585nm. Samples: Cell/tissue lysate, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.02 (OD), 0.002U/L (FL).

For quantitative determination of glucose-6-phosphate in biological samples. Key Features: Fast and sensitive. Linear detection range: 10 to 1000 µM G6P. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD460nm. Samples: Plasma, serum, tissue and culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 10 µM.

For quantitative determination of glutathione peroxidase activity and evaluation of drug effects on GPX activity. Key Features: Sensitive and accurate. Use 10 µL sample. Linear detection range 40 to 800 U/L GPX activity. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD340nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 40 U/L.

For quantitative determination of glutamate (monosodium glutamate or MSG) and evaluation of drug effects on glutamate metabolism. Key Features: Sensitive and accurate. Detection limit of 50 µM, linearity up to 2.5 mM glutamate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 30 min at room temperature. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, urine, cell, tissue etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 50 µM.

For quantitative determination of glutamine and evaluation of drug effects on glutamine metabolism. Key Features: Sensitive and accurate. Use 20 µL sample. Linear detection range 0.023 - 2 mM glutamine in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, incubation for 40 min at room temperature, adding a stop reagent and reading the optical density. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, urine, cell, tissue etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 23 µM.

For quantitative determination of glutathione reductase activity. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 50 U/L for 20 min reaction. Detection Limit of 0.4 U/L. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD412nm. Samples: Plasma, serum, tissue and culture media etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.4 U/L.

For quantitative determination of glycerol and evaluation of drug effects on glycerol metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 10 to 1000 µM (92 µg/dL to 9.2 mg/dL) glycerol for colorimetric assays and 2 to 50 µM for fluorimetric assays. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Method: OD570nm, or FL530/585nm. Samples: Biological, food, beverage etc. Species: All. Procedure: Assay takes 20 min. Kit size: 200 tests. Detection limit: 2 µM.

For quantitative determination of glycogen and evaluation of drug effects on glycogen metabolism. Key Features: Use as little as 10 µL samples. Linear detection range: 2 to 200 µg/mL glycogen for colorimetric assays and 0.2 to 20 µg/mL for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: OD, FL: 2, 0.2 µg/mL.

For quantitative determination of L-Lactate and screening for glycolysis modulators. Key Features: Fast and sensitive. Use of 5 µL sample. Linear detection range up to 10 mM L-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Cell media. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.

For quantitative determination of reduced/oxidized glutathione (GSH/GSSG) and evaluation of drug effects on their metabolism. Key Features: Sensitive and accurate. Linear detection range 0.01-3 µM GSH equivalents with a detection limit of 10 nM GSH equivalents. Method: OD412nm. Samples: Whole blood, plasma, serum, urine, tissue and cell extracts. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 10 nM.

For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism. Key Features: Sensitive and accurate. Requires only 20 µL serum sample. Detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay. Convenient. Room temperature assay. No 37°C heater is needed. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD340nm. Samples: Serum. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 5 mg/dL.

For quantitative determination of histamine in food, beverage and agricultural products. Key Features: Fast and sensitive. Use of 20 µL sample. Linear detection range 0.0025 - 1 mM (0.3 - 111 ppm) histamine in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Food, beverage and agricultural products. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2.5 µM.

Determination of intestinal permeability (leaky gut syndrome) through measuring lactulose/mannitol ratio.

For quantitative determination of invertase/sucrase enzyme activity. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.007 U/L invertase activity can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological, environment (soil) etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.007 U/L.

For quantitative colorimetric determination of isocitrate and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 20 to 5000 µM for 10 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.). Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 20 µM.

For quantitative determination of ketone body (acetoacetate and 3-hydroxybutyrate) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Uses 10 µL sample. Linear detection range of 0.12 to 8 mM for each ketone body in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at room temperature. High-throughput. Can be automated as a high-throughput 96-well plate assay for many samples per day. Method: OD340nm. Samples: Serum, plasma, urine etc. Species: All. Procedure: Assay takes 30 min. Kit size: 200 tests. Detection limit: 0.12 mM.

Rapid homogenous assay for protein kinase activity and high-throughput screen for kinase inhibitors. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.01 U/L kinase can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. The whole assay involves adding a single working reagent and incubation for 10 min at room temperature. Robust and amenable to HTS: Assay can tolerate up to 300 µM ATP and 10% dimethylsulfoxide (DMSO). Z factors of > 0.6 are routinely observed in 96/384-well plates. Can be readily automated on HTS liquid handling systems for tens of thousands of assays per day. Method: FL530/585nm. Samples: All kinases. Species: All. Procedure: Assay takes 10 min. Kit size: 400 tests. Detection limit: 0.01 U/L.

For quantitative determination of L-Alanine and evaluation of drug effects on alanine metabolism. Key Features: Sensitive and accurate. Linear detection range in 96-well plate: 1 to 200 µM alanine for colorimetric assays and 0.4 to 20 µM for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Plasma, serum, urine, tissue and culture media. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 0.4 µM.

For quantitative determination of L-Amino Acids in biological and other samples. Key Features: Fast and sensitive. Linear detection range: 3.3 to 500 µM (colorimetric assay) and 0.13 to 50 µM (fluorimetric assay) for 60 min reaction. Convenient. The procedure involves adding a single working reagent and reading after 60 minutes. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to process thousands of samples per day. Method: OD570nm or FL530/585nm. Samples: Serum, culture media, tissue homogenates, cell lysates, urine, food samples, etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 3.3 µM (colorimetric assay), 0.13 µM (fluorimetric assay).

For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.05 mM.

For quantitative determination of lactose and evaluation of drug effects on lactose metabolism. Key Features: Sensitive. Use as little as 20 µL samples. Linear detection range in 96-well plate: 17 to 2000 µM lactose for colorimetric assays and 6 to 100 µM for fluorimetric assays. Convenient and high-throughput. Robust and homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological (milk), food and agriculture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 6 µM.

For quantitative determination of lactulose in food, beverage, agricultural products, and biological samples

For quantitative determination of L-malate (L-malic acid) and drug effects on malate metabolism. Key Features: Fast and sensitive. Use of 20 µL sample. Linear detection range 0.02 to 2 mM L-malate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time 15 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Malate in food, juice, beverage and other agricultural products. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.02 mM.

For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 65 U/L for 20 min reaction at 37°C. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 U/L.

For quantitative determination of maltose and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 10 µL sample. Linear detection range 2 to 500 µM maltose for colorimetric assays and 1 to 50 µM for flourimetric assays. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, urine, food and beverages, etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 2 µM.

For rapid, quantitative determination of monoamine oxidase activity and MAO inhibitor screen. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.01 U/L MAO activity can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: MAO enzyme preparations. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.01 U/L.

For evaluation and high-throughput screen (HTS) of monoamine oxidase modulators. Key Features: Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: Monoamine Oxidase. Species: All. Procedure: Assay takes 35 min. Kit size: 100 tests.

For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 µM and linearity up to 10 µM NAD+/NADH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 15 min at room temperature. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Cell or tissue extracts. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.05 µM.

For quantitative determination of neuraminidase activity and screen for neuraminidase inhibitor. Key Features: Sensitive and accurate. Linear detection range at 37°C in 96-well plate: 0.1 to 10 U/L for colorimetric assays and 0.01 to 2 U/L for fluorimetric assays. Simple and convenient. Homogeneous assay requiring only two absorbance measurements. Assay can be completed in 60 min. High-throughput. Can be readily automated as a high-throughput 96-well plate assay to screen thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: OD, FL: 1, 0.01U/L.

For quantitative determination of nitric oxide synthase (NOS) activity and screen for NOS inhibitor. Key Features: Sensitive and accurate. Detection range 0.25 - 25 U/L in 96-well plate. Rapid and reliable. Can be completed in 40 min if reduction of NO₃^- to NO₂^- is performed at 60°C. Method: OD540nm. Samples: Biological. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.25 U/L.

For evaluation and high-throughput screen (HTS) of nitric oxide synthase (NOS) modulators. Key Features: High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in less than 3 hours if assay performed at 37°C. Method: OD540nm. Samples: Nitric Oxide Synthase. Species: All. Procedure: Assay takes 2-3 hours. Kit size: 100 tests.

For quantitative determination of oxalate (oxalic acid) in urine and other samples. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 10 minute incubation: 20 to 1500 µM oxalate. Fast and convenient. Sample pre-treatment is faster and easier than using activated carbon in competitor’s assay kits. High-throughput adaptable. The procedure involves addition of a single working reagent and incubation for 10 min at room temperature. Can be automated for processing thousands of samples per day. Method: OD595nm. Samples: urine, animal and plant tissue samples. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 20 µM.

For quantitative determination of oxaloacetate and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Linear detection range: 7 to 400 µM oxaloacetate for colorimetric assays and 1 to 40 µM for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Plasma, serum, tissue and culture media. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 4, 1 µM.

Determination of peanut in food and beverage samples.

For quantitative determination of L-phenylalanine and evaluation of drug effects on its metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. Linear detection range of 2 - 300 µM L-phenylalanine. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/595nm. Samples: Serum, urine etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2 µM.

For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism. Key Features: Sensitive. Use 10 µL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L. Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.04 U/L.

For quantitative determination of phospholipid and evaluation of drug effects on its metabolism. Key Features: Sensitive. Use 20 µL samples. Linear detection range: colorimetric assay 3 - 200 µM, fluorimetric assay 0.6 - 20 µM phospholipid. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 3, 0.6 µM.

For quantitative determination of pyruvate and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 2 to 500 µM (17 µg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 µM for fluorimetric assays. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 2, 0.2 µM.

For quantitative determination of pyruvate kinase (PK) activity and evaluation of drug effects on PK activity. Key Features: Sensitive and accurate. Linear detection range in 96-well plate: 0.1 to 50 U/L for colorimetric assays and 0.01 to 2 U/L for fluorimetric assays run at 25°C for 30 min. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, FL530/590nm. Samples: Plasma, serum, cell and tissue etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.01 U/L.

For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.02 to 1 mM sialic acid for colorimetric assays and 2 to 100 µM for fluorimetric assays. Simple and convenient. Can detect free sialic acid by addition of a single working reagent and incubation for 60 min at room temperature or total sialic acid by pre-treating samples with a 60 min hydrolysis step. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 tests. Detection limit: OD, FL: 20, 2 µM.

For quantitative determination of D-sorbitol and evaluation of drug effects on sorbitol metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 5 to 1000 µM D-sorbitol. Convenient and high-throughput. Room temperature assay. No 37°C incubator required. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological (e.g. blood), food, beverage and agriculture samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 5 µM.

For quantitative determination of starch and evaluation of drug effects on starch metabolism. Key Features: Use as little as 10 µL samples. Linear detection range: 2 to 200 µg/mL starch for colorimetric assays and 0.2 to 20 µg/mL for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Biological, agriculture, food etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2 µg/mL.

For quantitative determination of succinate (succinic acid) in food, beverage, agricultural products, and other biological samples. Key Features: Fast and sensitive. Use 20 µL sample. Linear detection range 10 to 400 µM for colorimetric assays and 2 to 40 µM for fluorimetric assays. Convenient. The procedure involves adding a single working reagent, and reading the absorbance or fluorescence after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD570nm, FL530/585nm. Samples: succinate in food, beverage, agricultural products, and other biological samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: Colorimetric assay: 10 µM; Fluorimetric assay: 2 µM.

For quantitative determination of sucrose.

For quantitative determination of superoxide dismutase (SOD) enzyme activity and evaluation of drug effects. Key Features: Sensitive and accurate. Linear detection range of 0.05 - 3 U/mL SOD. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD440nm. Samples: Blood, cell, tissue etc. Species: All. Procedure: Assay takes 1 hr. Kit size: 100 tests. Detection limit: 0.05 U/mL.

For quantitative determination of triglyceride and evaluation of drug effects on triglyceride metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range 0.01 to 1.0 mmol/L (0.88 to 88.5 mg/dL) triglyceride. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Method: OD570nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 30 min. Kit size: 200 tests. Detection limit: 0.01 mmol/L (0.88 mg/dL).

For quantitative enzymatic determination of urea. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 50 to 1000 µM urea in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay.Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD340nm. Samples: Urea in biological samples (e.g. plasma, serum, urine, bronchoalveolar lavage (BAL)) and food/beverage samples (e.g. milk). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 50 µM.

For quantitative determination of xanthine and drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 30 minute incubation: 0.01 to 2.5 mM xanthine for colorimetric assays and 3 to 250 µM for fluorimetric assays. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature. Fast and high-throughput. Assays using 96-well plates and liquid handling system could allow simultaneous processing tens of thousands of samples per day. Method: OD570nm, FL530/585nm. Samples: Cell lysate, serum, and other biological samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: FL 3 µM; OD 10 µM.

For quantitative determination of xanthine oxidase enzyme activity and evaluation of its modulators. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 20 minute incubation: 0.03 to 25 U/L xanthine oxidase for colorimetric assays and 0.01 to 2.5 U/L for fluorimetric assays. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Method: OD570nm, FL530/585nm. Samples: Cell lysate, serum, and other biological samples. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: FL 0.01 U/L; OD 0.03 U/L.