EnzyChrom™ L-Amino Acid Kit

Application

• Quantitative determination of L-Amino Acids in biological and other samples.

Key Features

• Fast and sensitive. Linear detection range: 3.3 to 500 µM (colorimetric assay) and 0.13 to 50 µM (fluorimetric assay) for 60 min reaction.
• Convenient. The procedure involves adding a single working reagent and reading after 60 minutes.
• High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to process thousands of samples per day.

Method

•  OD570nm or FL530/585nm

Samples

•  Serum, culture media, tissue homogenates, cell lysates, urine, food samples, etc.

Species

•  All

Size

•  100 tests

Detection Limit

•  3.3 μM (colorimetric assay), 0.13 μM (fluorimetric assay)

Shelf Life

•  6 months

More Details

•  L-AMINO-ACIDS are the building blocks of proteins in biology. Almost all of the common 20 amino acids exist as the L-enantiomer. BioAssay Systems' L-amino acid assay uses an enzyme-catalyzed oxidation of Lamino acids to convert a dye into a colored and fluorescent form. The absorbance at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the L-amino acid concentration in the sample.