HTS Reagents & Kits

17 Product(s)
  1. CellQuanti-Blue™ Cell Viability Assay Kits (CQBL)

    Starting at: £186.15

    Homogeneous assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents.
  2. CellQuanti-MTT™ Cell Viability Assay Kits (CQMT-500)

    £186.15
    Product Size: 500 Tests
    Homogeneous assay for cell viability, proliferation, cytotoxcity and evaluation of anticancer agents. Key Features: Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 950 cells can be accurately quantified. Fast. High-throughput assay using 96-well plates allows simultaneous processing tens of thousands of samples per day. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS. Z factors of 0.5 and above are observed. Can be readily automated with HTS liquid handling systems. Method: OD570nm. Samples: Cell culture. Species: All. Procedure: Assay takes 5 hrs, hands-on time 30 min. Kit size: 500 tests. Detection limit: 950 cells.
  3. EnzyChrom™ Adipolysis Assay Kit (EAPL-200)

    £377.65
    Product Size: 200 Tests
    For quantitative assay of adipolysis and evaluation of drug effects on adipolysis. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.92 to 100 µg/mL (10 to 1000 µM) glycerol for colorimetric assays and 0.2 to 5 µg/mL for fluorimetric assays. Rapid and convenient.The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates. Method: OD570nm, or FL530/585nm. Samples: Cell culture media. Species: All. Procedure: Assay takes 20 min. Kit size: 200 tests. Detection limit: 0.92 µg/mL.
  4. EnzyChrom™ Kinase Assay Kit (EKIN-400)

    £292.65
    Product Size: 400 Tests
    Rapid homogenous assay for protein kinase activity and high-throughput screen for kinase inhibitors. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.01 U/L kinase can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. The whole assay involves adding a single working reagent and incubation for 10 min at room temperature. Robust and amenable to HTS: Assay can tolerate up to 300 µM ATP and 10% dimethylsulfoxide (DMSO). Z factors of > 0.6 are routinely observed in 96/384-well plates. Can be readily automated on HTS liquid handling systems for tens of thousands of assays per day. Method: FL530/585nm. Samples: All kinases. Species: All. Procedure: Assay takes 10 min. Kit size: 400 tests. Detection limit: 0.01 U/L.
  5. EnzyChrom™ Neuraminidase Assay Kit (ENEU-100)

    £488.15
    Product Size: 100 Tests
    For quantitative determination of neuraminidase activity and screen for neuraminidase inhibitor. Key Features: Sensitive and accurate. Linear detection range at 37°C in 96-well plate: 0.1 to 10 U/L for colorimetric assays and 0.01 to 2 U/L for fluorimetric assays. Simple and convenient. Homogeneous assay requiring only two absorbance measurements. Assay can be completed in 60 min. High-throughput. Can be readily automated as a high-throughput 96-well plate assay to screen thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: OD, FL: 1, 0.01U/L.
  6. EnzyFluo™ Collagen Assay Kit (ECOL-100)

    £420.15
    Product Size: 100 Tests
    For quantitatve determination of collagen in biological and cosmetic products. Key Features: Safe. Non-radioactive assay. Fast and sensitive. Linear detection range (20µL sample): 2 µg/mL to 50µg/mLcollagen in 96-well plate assay. Convenient and high-throughput."Add-mix-read" type assay. No wash and reagent transfer steps are involved. Can be readily automated for processing thousands of samples per day. Method: FL375/465nm. Samples: Biological samples (e.g. plasma, serum, cells) and cosmetic samples (e.g. collagen solution). Species: All. Procedure: Assay takes 80 min. Kit size: 100 tests. Detection limit: 2 µg/mL.
  7. EnzyLight™ Cytotoxicity Assay Kit (ECTX-100)

    £292.65
    Product Size: 100 Tests
    For rapid, bioluminescent luciferin/luciferase based assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents. Key Features: Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 50 cells can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.6 to 0.7 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Luminescence. Samples: Cells. Species: All. Procedure: Assay takes 2 min. Kit size: 100 tests. Detection limit: 50 cells.
  8. LDH Cytotoxicity Assay Kit (C2LD-100)

    £109.65
    Product Size: 100 Tests
    For rapid quantitative determination of cytotoxicity based on lactate dehydrogenase released into cell culture medium. Evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical treatment on cell lysis. Key Features: Safe. Non-radioactive assay (cf. chromium release assay). Fast. The whole procedure take 20 min. Robust and amenable to HTS. Single reagent, "mix-incubate-measure" type assay. High-throughput assay in 96-well plates allows simultaneous processing tens of thousands of samples per day. Method: OD500nm. Samples: Cell culture. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests.
  9. Malachite Green Phosphate Assay Kit (POMG-25H)

    £237.15
    Product Size: 2500 Tests
    For sensitive and high-throughput phosphate determination. Key Features: Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 1.6 pmoles of phosphate and useful range between 0.02 µM and 40 µM phosphate. Fast and convenient: homogeneous "mix-and-measure" assay allows quantitation of free phosphate within 20 minutes. Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: OD620nm (malachite green). Samples: Biological, environment etc. Species: All. Procedure: Assay takes 30 min. Kit size: 2500 tests. Detection limit: 0.02 µM.
  10. Parallel Artificial Membrane Permeability Assay (PAMPA) Kit (PAMPA-096)

    £373.15
    Product Size: 96 Tests
    For quantitative determination of gastrointestinal (GI) membrane permeability of test compounds. Key Features: Convenient. Includes all necessary equipment to run a PAMPA plate. Simple and low-cost. Procedure is easy to follow and more affordable than cell-based permeability assays. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: PAMPA. Samples: Chemical compounds. Species: All. Procedure: Assay takes 20 hr, hands-on time 1 hr. Kit size: 96 tests.
  11. Parallel Artificial Membrane Permeability Assay-BBB Kit (PMBBB-096)

    £373.15
    Product Size: 96 Tests
    For quantitative determination of blood brain barrier (BBB) membrane permeability of test compounds. Key Features: Convenient. Includes all necessary equipment to run a PAMPA plate. Simple and low-cost. Procedure is easy to follow and more affordable than cell-based permeability assays. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: PAMPA. Samples: Chemical compounds. Procedure: Assay takes 20 hr, hands-on time 1 hr. Kit size: 96 tests.
  12. Parallel Artificial Membrane Permeability Assay-Skin Kit (PMSKN-096)

    £373.15
    Product Size: 96 Tests
    For quantitative determination of skin membrane permeability of test compounds. Key Features: Convenient. Includes all necessary equipment to run a PAMPA plate. Simple and low-cost. Procedure is easy to follow and more affordable than cell-based permeability assays. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: PAMPA. Samples: Chemical Compounds. Procedure: Assay takes 20 hrs, hands-on time 1 hr. Kit size: 96 tests.
  13. QuantiChrom™ Biotin Assay Kit (DBIO-100)

    £203.15
    Product Size: 100 Tests
    For quantitative determination of biotin in food, cosmetic, and biotinylated proteins. Key Features: Fast and sensitive. Linear detection range: 8 to 200 µM biotin with 30 µL sample (96-well) or 10 to 200 µM biotin with 10 µL sample (384-well). Convenient. The procedure involves adding a single working reagent and reading after 20 minutes. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD500nm. Samples: Food, cosmetics, supplements, and biotinylated proteins or antibodies. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 8 µM.
  14. QuantiChrom™ Fatty Acid Uptake Assay Kit (DFFU-100)

    £381.65
    Product Size: 100 Tests
    For quantitative determination of long-chain fatty acid uptake in whole cells and evaluation of effects of ligands or drugs on fatty acid transport. Key Features: Safe. Non-radioactive assay. Fast and Sensitive. Homogenous "add-and-read" assay. No wash, lysis, or staining steps needed. Simple and Convenient. Can be automated as a high-throughput assay for fatty acid transport and modulator screens in cells. Method: FL 488/523nm. Samples: Adipocytes and other fatty acid transporting cells. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 Tests.
  15. QuantiChrom™ Indole Assay Kit (DIND-100)

    £177.65
    Product Size: 100 Tests
    For quantitative determination of indole and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 100µL sample. Linear detection range from 3 to 100 µM indole in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance immediately. Method: OD565nm. Samples: Bacterial growth media and other biological samples. Species: All. Procedure: Assay takes 5 min. Kit size: 100 tests. Detection limit: 3 µM.
  16. SuperLight™ Dual Luciferase Reporter Gene Assay Kit (SLDL-100)

    £169.15
    Product Size: 100 Tests
    Bioluminescent reagent system for rapid quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells. Key Features: High sensitivity and wide detection range. Detection of as little of 2 fg luciferase. Compatible with routine laboratory and HTS formats. Assays can be performed in tubes or microplates, and measured with any luminometer. Can be readily automated on HTS liquid handling systems. Fast and convenient. Three step assay allows detection of dual luciferase levels within 20 minutes. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 2 fg luciferase.
  17. SuperLight™ Luciferase Reporter Gene Assay Kit (SLLU)

    Starting at: £152.15

    Bright bioluminescent reagent system for rapid quantitation of luciferase reporter gene expression in transfected cells and high-throughput drug screens.