EnzyChrom™ Adipolysis Assay KitEnzyChrom™ Adipolysis Assay Kit
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EnzyChrom™ Adipolysis Assay Kit

For quantitative assay of adipolysis and evaluation of drug effects on adipolysis.

• Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 0.92 to 100 μg/mL (10 to 1000 μM) glycerol for colorimetric assays and 0.2 to 5 μg/mL for fluorimetric assays.

• Rapid and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature.

• Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates. 

Obesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue. The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. ADIPOLYSIS or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery. BioAssay Systems’ adipolysis assay kit directly measures glycerol released during adipolysis. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm is directly proportional to glycerol concentration in the sample.

Cat# Size Price Qty Buy
EAPL-200 200 Tests £301.15

Additional Information

Property Value or Rating
Product Size 200 Tests
Manufacturer BioAssay Systems
Applications For quantitative assay of adipolysis and evaluation of drug effects on adipolysis.
Method OD570nm, or FL530/585nm
Samples Cell culture media
Species All
Detection Limit 0.92 μg/mL
Storage -20°C
Shelf Life 12 months
References Assay: Adipolysis in Mouse 3T3-L1 adipocytes (Pubmed).

2. Huttala, O et al (2016). Differentiation of human adipose stromal cells in vitro into insulin-sensitive adipocytes. Cell and Tissue Research 1-12. Assay: Cells in Humans (Pubmed).

3. Kim, HK et al (2016). An apolipoprotein B100 mimotope prevents obesity in mice. Clinical Science 130(2): 105-16. Assay: Adipocytes in Mice (Pubmed).

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1. Li R et al (2012). Neuropeptide Y potentiates beta-adrenergic stimulation of lipolysis in 3T3-L1 adipocytes. Regul Pept. 178(1-3):16-20. Assay: Adipolysis in Mouse 3T3-L1 adipocytes (Pubmed).

2. Huttala, O et al (2016). Differentiation of human adipose stromal cells in vitro into insulin-sensitive adipocytes. Cell and Tissue Research 1-12. Assay: Cells in Humans (Pubmed).

3. Kim, HK et al (2016). An apolipoprotein B100 mimotope prevents obesity in mice. Clinical Science 130(2): 105-16. Assay: Adipocytes in Mice (Pubmed).

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1. Li R et al (2012). Neuropeptide Y potentiates beta-adrenergic stimulation of lipolysis in 3T3-L1 adipocytes. Regul Pept. 178(1-3):16-20. Assay: Adipolysis in Mouse 3T3-L1 adipocytes (Pubmed).

2. Huttala, O et al (2016). Differentiation of human adipose stromal cells in vitro into insulin-sensitive adipocytes. Cell and Tissue Research 1-12. Assay: Cells in Humans (Pubmed).

3. Kim, HK et al (2016). An apolipoprotein B100 mimotope prevents obesity in mice. Clinical Science 130(2): 105-16. Assay: Adipocytes in Mice (Pubmed).

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