EnzyChrom™ Glutamate Assay Kit

• Sensitive and accurate. Detection limit of 50 μM, linearity up to 2.5 mM glutamate in 96-well plate assay.

• Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 30 min at room temperature. No 37°C heater is needed. 

• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Glutamate is an important chemical in general metabolism. It is also a crucial mammalian neurotransmitter that is believed to be involved in a number of neurological and psychiatric disorders such as lateral sclerosis, lathyrism, autism and Alzheimer’s disease. Glutamate is also widely used as a flavor enhancer in the food industry. Simple, direct and automation-ready procedures for measuring glutamate concentration are very desirable. BioAssay Systems’ EnzyChrom™ glutamate assay kit is based on glutamate dehydrogenase catalyzed oxidation of glutamate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the glutamate concentration in the sample.