EnzyChrom™ Lactate Assay KitEnzyChrom™ Lactate Assay Kit
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EnzyChrom™ Lactate Assay Kit

For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.

• Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay.

• Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed. 

• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems’ EnzyChrom™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

Cat# Size Price Qty Buy
ECLC-100 100 Tests £326.65

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.
Method OD565nm
Samples Serum, plasma, cell culture media etc
Species All
Detection Limit 0.05 mM
Storage -20°C
Shelf Life 6 months
References Assay: L-Lactate in Mouse Plasma (Pubmed).

2. Zheng, Y et al (2009). Anergic T cells are metabolically anergic. J Immunol 183(10):6095-101. Assay: L-Lactate in Mouse Cell culture medium (T cells) (Pubmed).

3. Le Nihouannen, D et al (2009). Ascorbic acid accelerates osteoclast formation and death. Bone 46(5):1336-43. Assay: L-Lactate in Mouse Cell culture medium (RAW 264.7 monocytic cell line) (Pubmed).

4. Fan, Y et al (2009). Akt and c-Myc differentially activate cellular metabolic programs and prime cells to bioenergetic inhibition. J Biol Chem 285(10):7324-33. Assay: L-Lactate in Mouse Cell culture medium (FL5.12 pre B-cell line) (Pubmed).

5. Wei, S et al (2010). Energy restriction as an antitumor target of thiazolidinediones. J Biol Chem 285(13):9780-91. Assay: L-Lactate in Human LNCaP cells (Pubmed).

6. Toschi, A et al (2010). Phospholipase D-mTOR requirement for the Warburg effect in human cancer cells. Cancer Lett 299(1):72-9. Assay: L-Lactate in Human (renal and breast cancer cells) (Pubmed).

7. Milovanova, TN et al (2009). Hyperbaric oxygen stimulates vasculogenic stem cell growth and differentiation in vivo. J Appl Physiol 106(2):711-28. Assay: L-Lactate in N/A Matrigel plugs (Pubmed).

8. Devalaraja-Narashimha K, Padanilam BJ (2009). PARP-1 inhibits glycolysis in ischemic kidneys. J Am Soc Nephrol 20(1):95-103. Assay: L-Lactate in Mouse Kidney (Pubmed).

9. Kashimshetty, R et al (2009). Underlying mitochondrial dysfunction triggers flutamide-induced oxidative liver injury in a mouse model of idiosyncratic drug toxicity. Toxicol Appl Pharmacol 238(2):150-9. Assay: L-Lactate in Mouse Serum (Pubmed).

10. Weber, J et al (2009). Hyperalgesia induced by oral stavudine administration to rats does not depend on spinal neuronal cell death, or on spinal or systemic inflammatory cytokine secretion, or metabolic dysregulation. Neurotoxicology 30(3):423-9. Assay: L-Lactate in Rat Plasma (Pubmed).

11. Roy, SG et al (2009). Excess of glucocorticoid induces cardiac dysfunction via activating angiotensin II pathway. Cell Physiol Biochem 24(1-2):1-10. Assay: L-Lactate in Rat Serum (Pubmed).

12. Senadheera, D et al (2009). Inactivation of VicK affects acid production and acid survival of Streptococcus mutans. J Bacteriol 191(20):6415-24. Assay: L-Lactate in Streptococcus mutans Bacteria (Pubmed).

13. Krastel, K et al (2010). Characterization of a glutamate transporter operon, glnQHMP, in Streptococcus mutans and its role in acid tolerance. J Bacteriol 192(4):984-93. Assay: L-Lactate in Streptococcus mutans Bacteria culture supernatant (Pubmed).

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1. Aronoff, DM et al (2009). E-prostanoid 3 receptor deletion improves pulmonary host defense and protects mice from death in severe Streptococcus pneumoniae infection. J Immunol 183(4):2642-9. Assay: L-Lactate in Mouse Plasma (Pubmed).

2. Zheng, Y et al (2009). Anergic T cells are metabolically anergic. J Immunol 183(10):6095-101. Assay: L-Lactate in Mouse Cell culture medium (T cells) (Pubmed).

3. Le Nihouannen, D et al (2009). Ascorbic acid accelerates osteoclast formation and death. Bone 46(5):1336-43. Assay: L-Lactate in Mouse Cell culture medium (RAW 264.7 monocytic cell line) (Pubmed).

4. Fan, Y et al (2009). Akt and c-Myc differentially activate cellular metabolic programs and prime cells to bioenergetic inhibition. J Biol Chem 285(10):7324-33. Assay: L-Lactate in Mouse Cell culture medium (FL5.12 pre B-cell line) (Pubmed).

5. Wei, S et al (2010). Energy restriction as an antitumor target of thiazolidinediones. J Biol Chem 285(13):9780-91. Assay: L-Lactate in Human LNCaP cells (Pubmed).

6. Toschi, A et al (2010). Phospholipase D-mTOR requirement for the Warburg effect in human cancer cells. Cancer Lett 299(1):72-9. Assay: L-Lactate in Human (renal and breast cancer cells) (Pubmed).

7. Milovanova, TN et al (2009). Hyperbaric oxygen stimulates vasculogenic stem cell growth and differentiation in vivo. J Appl Physiol 106(2):711-28. Assay: L-Lactate in N/A Matrigel plugs (Pubmed).

8. Devalaraja-Narashimha K, Padanilam BJ (2009). PARP-1 inhibits glycolysis in ischemic kidneys. J Am Soc Nephrol 20(1):95-103. Assay: L-Lactate in Mouse Kidney (Pubmed).

9. Kashimshetty, R et al (2009). Underlying mitochondrial dysfunction triggers flutamide-induced oxidative liver injury in a mouse model of idiosyncratic drug toxicity. Toxicol Appl Pharmacol 238(2):150-9. Assay: L-Lactate in Mouse Serum (Pubmed).

10. Weber, J et al (2009). Hyperalgesia induced by oral stavudine administration to rats does not depend on spinal neuronal cell death, or on spinal or systemic inflammatory cytokine secretion, or metabolic dysregulation. Neurotoxicology 30(3):423-9. Assay: L-Lactate in Rat Plasma (Pubmed).

11. Roy, SG et al (2009). Excess of glucocorticoid induces cardiac dysfunction via activating angiotensin II pathway. Cell Physiol Biochem 24(1-2):1-10. Assay: L-Lactate in Rat Serum (Pubmed).

12. Senadheera, D et al (2009). Inactivation of VicK affects acid production and acid survival of Streptococcus mutans. J Bacteriol 191(20):6415-24. Assay: L-Lactate in Streptococcus mutans Bacteria (Pubmed).

13. Krastel, K et al (2010). Characterization of a glutamate transporter operon, glnQHMP, in Streptococcus mutans and its role in acid tolerance. J Bacteriol 192(4):984-93. Assay: L-Lactate in Streptococcus mutans Bacteria culture supernatant (Pubmed).

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1. Aronoff, DM et al (2009). E-prostanoid 3 receptor deletion improves pulmonary host defense and protects mice from death in severe Streptococcus pneumoniae infection. J Immunol 183(4):2642-9. Assay: L-Lactate in Mouse Plasma (Pubmed).

2. Zheng, Y et al (2009). Anergic T cells are metabolically anergic. J Immunol 183(10):6095-101. Assay: L-Lactate in Mouse Cell culture medium (T cells) (Pubmed).

3. Le Nihouannen, D et al (2009). Ascorbic acid accelerates osteoclast formation and death. Bone 46(5):1336-43. Assay: L-Lactate in Mouse Cell culture medium (RAW 264.7 monocytic cell line) (Pubmed).

4. Fan, Y et al (2009). Akt and c-Myc differentially activate cellular metabolic programs and prime cells to bioenergetic inhibition. J Biol Chem 285(10):7324-33. Assay: L-Lactate in Mouse Cell culture medium (FL5.12 pre B-cell line) (Pubmed).

5. Wei, S et al (2010). Energy restriction as an antitumor target of thiazolidinediones. J Biol Chem 285(13):9780-91. Assay: L-Lactate in Human LNCaP cells (Pubmed).

6. Toschi, A et al (2010). Phospholipase D-mTOR requirement for the Warburg effect in human cancer cells. Cancer Lett 299(1):72-9. Assay: L-Lactate in Human (renal and breast cancer cells) (Pubmed).

7. Milovanova, TN et al (2009). Hyperbaric oxygen stimulates vasculogenic stem cell growth and differentiation in vivo. J Appl Physiol 106(2):711-28. Assay: L-Lactate in N/A Matrigel plugs (Pubmed).

8. Devalaraja-Narashimha K, Padanilam BJ (2009). PARP-1 inhibits glycolysis in ischemic kidneys. J Am Soc Nephrol 20(1):95-103. Assay: L-Lactate in Mouse Kidney (Pubmed).

9. Kashimshetty, R et al (2009). Underlying mitochondrial dysfunction triggers flutamide-induced oxidative liver injury in a mouse model of idiosyncratic drug toxicity. Toxicol Appl Pharmacol 238(2):150-9. Assay: L-Lactate in Mouse Serum (Pubmed).

10. Weber, J et al (2009). Hyperalgesia induced by oral stavudine administration to rats does not depend on spinal neuronal cell death, or on spinal or systemic inflammatory cytokine secretion, or metabolic dysregulation. Neurotoxicology 30(3):423-9. Assay: L-Lactate in Rat Plasma (Pubmed).

11. Roy, SG et al (2009). Excess of glucocorticoid induces cardiac dysfunction via activating angiotensin II pathway. Cell Physiol Biochem 24(1-2):1-10. Assay: L-Lactate in Rat Serum (Pubmed).

12. Senadheera, D et al (2009). Inactivation of VicK affects acid production and acid survival of Streptococcus mutans. J Bacteriol 191(20):6415-24. Assay: L-Lactate in Streptococcus mutans Bacteria (Pubmed).

13. Krastel, K et al (2010). Characterization of a glutamate transporter operon, glnQHMP, in Streptococcus mutans and its role in acid tolerance. J Bacteriol 192(4):984-93. Assay: L-Lactate in Streptococcus mutans Bacteria culture supernatant (Pubmed).

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