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EnzyChrom™ α-Amylase Assay KitEnzyChrom™ α-Amylase Assay Kit
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EnzyChrom™ α-Amylase Assay Kit

For quantitative determination of α-amylase activity.

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• Sensitive and accurate. Linear detection range 0.3 to 50 U/L α-amylase in 96-well plate assay.

• Convenient. The procedure involves adding a single working reagent, incubation for 15 min, followed by the detection reagent and a 20-min incubation and reading the optical density at 585 nm.

AMYLASE belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4-glycosidic bonds. The α-amylases (EC 3.2.1.1) cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose and "limit dextrin" from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis and renal failure. Simple, direct and automation-ready procedures for measuring amylase activity are very desirable. BioAssay Systems’ EnzyChrom™ a-amylase assay method involves two steps: (1). α-amylase in the sample hydrolyzes starch and the product is rapidly converted to glucose by α-glucosidase and hydrogen peroxide by glucose oxidase; (2). hydrogen peroxide concentration is determined with a colorimetric reagent.

Cat# Size Price Qty Buy
ECAM-100 100 Tests £360.65

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of α-amylase activity.
Method OD585nm
Samples Blood, saliva, urine, agriculture etc 
Species All
Detection Limit 0.3 U/L
Storage -20°C
Shelf Life 6 months
References Assay: a-Amylase in Human Saliva.

2. S. B. Lee et al (2013). Effect of Oral Administration of Intact Casein on Gastrointestinal Hormone Secretion and Pancreatic ?-Amylase Activity in Korean Native Steer. Asian-Australasian Journal of Animal Sciences (AJAS). 26(5): 654-660. Assay: a-Amylase in steers (cow) pancreas (duodenal fluid).

3. Lee, Kyung-Hoon, et al.(2014). Identification of proteins involved in the pancreatic exocrine by exogenous ghrelin administration in Sprague-Dawley rats. J. Animal Sci. Techn. 56.1 : 6. Assay: a-Amylase in Rat plasma.

4. Abu Khadra, KM et al (2014)/ Antioxidant Profile of Saliva among Young Men Using Mobile Phones. Jordan Journal of Biological Sciences. 7(4):275-280.

5.Han, Mee-Jung, and Seung Hwan Lee (2015). An efficient bacterial surface display system based on a novel outer membrane anchoring element from the Escherichia coli protein YiaT. FEMS Microbiol. Lett. 362.1: 1-7. Assay: a-Amylase in Bacillus subtilis (bacteria) cells.

6. "Lee, KH et al (2014). Identification of proteins involved in the pancreatic exocrine by exogenous ghrelin administration in Sprague-Dawley rats. Journal of Animal Science and Technology. 56:6 Assay: Pancreas Tissue in Sprague-Dawley rats (Pubmed).

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1. Mohannad N et al (2014). Age-related changes in salivary biomarkers. Journal of Dental Sciences. 9(1): 85-90. Assay: a-Amylase in Human Saliva.

2. S. B. Lee et al (2013). Effect of Oral Administration of Intact Casein on Gastrointestinal Hormone Secretion and Pancreatic ?-Amylase Activity in Korean Native Steer. Asian-Australasian Journal of Animal Sciences (AJAS). 26(5): 654-660. Assay: a-Amylase in steers (cow) pancreas (duodenal fluid).

3. Lee, Kyung-Hoon, et al.(2014). Identification of proteins involved in the pancreatic exocrine by exogenous ghrelin administration in Sprague-Dawley rats. J. Animal Sci. Techn. 56.1 : 6. Assay: a-Amylase in Rat plasma.

4. Abu Khadra, KM et al (2014)/ Antioxidant Profile of Saliva among Young Men Using Mobile Phones. Jordan Journal of Biological Sciences. 7(4):275-280.

5.Han, Mee-Jung, and Seung Hwan Lee (2015). An efficient bacterial surface display system based on a novel outer membrane anchoring element from the Escherichia coli protein YiaT. FEMS Microbiol. Lett. 362.1: 1-7. Assay: a-Amylase in Bacillus subtilis (bacteria) cells.

6. "Lee, KH et al (2014). Identification of proteins involved in the pancreatic exocrine by exogenous ghrelin administration in Sprague-Dawley rats. Journal of Animal Science and Technology. 56:6 Assay: Pancreas Tissue in Sprague-Dawley rats (Pubmed).

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1. Mohannad N et al (2014). Age-related changes in salivary biomarkers. Journal of Dental Sciences. 9(1): 85-90. Assay: a-Amylase in Human Saliva.

2. S. B. Lee et al (2013). Effect of Oral Administration of Intact Casein on Gastrointestinal Hormone Secretion and Pancreatic ?-Amylase Activity in Korean Native Steer. Asian-Australasian Journal of Animal Sciences (AJAS). 26(5): 654-660. Assay: a-Amylase in steers (cow) pancreas (duodenal fluid).

3. Lee, Kyung-Hoon, et al.(2014). Identification of proteins involved in the pancreatic exocrine by exogenous ghrelin administration in Sprague-Dawley rats. J. Animal Sci. Techn. 56.1 : 6. Assay: a-Amylase in Rat plasma.

4. Abu Khadra, KM et al (2014)/ Antioxidant Profile of Saliva among Young Men Using Mobile Phones. Jordan Journal of Biological Sciences. 7(4):275-280.

5.Han, Mee-Jung, and Seung Hwan Lee (2015). An efficient bacterial surface display system based on a novel outer membrane anchoring element from the Escherichia coli protein YiaT. FEMS Microbiol. Lett. 362.1: 1-7. Assay: a-Amylase in Bacillus subtilis (bacteria) cells.

6. "Lee, KH et al (2014). Identification of proteins involved in the pancreatic exocrine by exogenous ghrelin administration in Sprague-Dawley rats. Journal of Animal Science and Technology. 56:6 Assay: Pancreas Tissue in Sprague-Dawley rats (Pubmed).

Related Documents

1. Mohannad N et al (2014). Age-related changes in salivary biomarkers. Journal of Dental Sciences. 9(1): 85-90. Assay: a-Amylase in Human Saliva.

2. S. B. Lee et al (2013). Effect of Oral Administration of Intact Casein on Gastrointestinal Hormone Secretion and Pancreatic ?-Amylase Activity in Korean Native Steer. Asian-Australasian Journal of Animal Sciences (AJAS). 26(5): 654-660. Assay: a-Amylase in steers (cow) pancreas (duodenal fluid).

3. Lee, Kyung-Hoon, et al.(2014). Identification of proteins involved in the pancreatic exocrine by exogenous ghrelin administration in Sprague-Dawley rats. J. Animal Sci. Techn. 56.1 : 6. Assay: a-Amylase in Rat plasma.

4. Abu Khadra, KM et al (2014)/ Antioxidant Profile of Saliva among Young Men Using Mobile Phones. Jordan Journal of Biological Sciences. 7(4):275-280.

5.Han, Mee-Jung, and Seung Hwan Lee (2015). An efficient bacterial surface display system based on a novel outer membrane anchoring element from the Escherichia coli protein YiaT. FEMS Microbiol. Lett. 362.1: 1-7. Assay: a-Amylase in Bacillus subtilis (bacteria) cells.

6. "Lee, KH et al (2014). Identification of proteins involved in the pancreatic exocrine by exogenous ghrelin administration in Sprague-Dawley rats. Journal of Animal Science and Technology. 56:6 Assay: Pancreas Tissue in Sprague-Dawley rats (Pubmed).

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