EnzyChrom™ Phospholipase D Assay KitEnzyChrom™ Phospholipase D Assay Kit
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EnzyChrom™ Phospholipase D Assay Kit

For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.

• Sensitive. Use 10 μL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L.

• Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening.

Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers. BioAssay Systems’ method provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.

Cat# Size Price Qty Buy
EPPD-100 100 Tests £275.65

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.
Method OD570nm, or FL530/585nm
Samples Biological
Species All
Detection Limit 0.04 U/L
Storage -20°C
Shelf Life 6 months
References Assay: Phospholipase D in Rat tissue (Pubmed).

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1. Zhu X et al (2014). The role of phospholipase D1 in liver fibrosis induced by dimethylnitrosamine in vivo. Dig Dis Sci. 59(8):1779-88. Assay: Phospholipase D in Rat tissue (Pubmed).

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1. Zhu X et al (2014). The role of phospholipase D1 in liver fibrosis induced by dimethylnitrosamine in vivo. Dig Dis Sci. 59(8):1779-88. Assay: Phospholipase D in Rat tissue (Pubmed).

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