EnzyChrom™ Sorbitol Assay KitEnzyChrom™ Sorbitol Assay Kit
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EnzyChrom™ Sorbitol Assay Kit

For quantitative determination of D-sorbitol and evaluation of drug effects on sorbitol metabolism.

• Fast and sensitive. Linear detection range (20 μL sample): 5 to 1000 μM D-sorbitol. 

• Convenient and high-throughput. Room temperature assay. No 37°C incubator required. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. 

The mitogen-activated protein kinase (MAPK/ERK) pathway plays a key role in cell proliferation, differentiation and migration. Stimulation by mitogens eventually leads to phosphorylation of ERK1 (T202/Y204) and ERK2 (T185/Y187). The MAPK/ERK cascade presents many interesting drug targets for the development of cancer therapies. BioAssay Systems’ cell-based ELISA measures dually phosphorylated ERK1/2 in whole cells. This simple and efficient assay eliminates the need for cell lysate preparation and normalizes the signal to the total protein content. The assay is a useful tool to study the MAP kinase signaling pathway. In this assay, cells are grown in 96-well plates and treated with ligands or drugs. Cells are then fixed and permeabilized in the wells. ERK1/2 phosphorylation (pERK) is measured using a HRP-conjugated antibody specific for phosphorylated ERK1/2.

Cat# Size Price Qty Buy
ESBT-100 100 Tests £343.65

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of D-sorbitol and evaluation of drug effects on sorbitol metabolism.
Method OD565nm
Samples Biological (e.g. blood), food, beverage and agriculture samples. 
Species All
Detection Limit 5 μM
Storage -20°C
Shelf Life 6 months

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