EnzyChrom™ Glycerol Assay KitEnzyChrom™ Glycerol Assay Kit
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EnzyChrom™ Glycerol Assay Kit

For quantitative determination of glycerol and evaluation of drug effects on glycerol metabolism.

• Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 10 to 1000 μM (92 μg/dL to 9.2 mg/dL) glycerol for colorimetric assays and 2 to 50 μM for fluorimetric assays.

• Simple and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature, compatible for HTS assays. 

• Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. 

Glycerol [Glycerin or Glycerine, C3H5 (OH) 3] is widely used in foods, beverages and pharmaceutical formulations. It is also a main by-product of biodiesel production. Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide applications. BioAssay Systems’ glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to glycerol concentration in the sample.

Cat# Size Price Qty Buy
EGLY-200 200 Tests £301.15

Additional Information

Property Value or Rating
Product Size 200 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of glycerol and evaluation of drug effects on glycerol metabolism.
Method OD570nm, or FL530/585nm
Samples Biological, food, beverage etc
Species All
Detection Limit 2 μM
Storage -20°C
Shelf Life 12 months
References Assay: Glycerol in N/A Diacylglycerol (Pubmed).

2. Drew, BG et al (2011). Reconstituted high-density lipoprotein infusion modulates fatty acid metabolism in patients with type 2 diabetes mellitus. J Lipid Res 52(3):572-581. Assay: Glycerol in Human Plasma (Pubmed).

3. Wachtler, B et al (2011). From attachment to damage: defined genes of Candida albicans mediate adhesion, invasion and damage during interaction with oral epithelial cells. PLoS One 6(2):e17046. Assay: Glycerol in Fungi C. albicans (Pubmed).

4. Bahar, B et al. A potential role of IL-6 in the chito-oligosaccharide-mediated inhibition of adipogenesis (2011). Br J Nutr. 106(8):1142-1153. Assay: Glycerol in Mouse 3T3-L1 cells (Pubmed).

5. . Assay: Glycerol in Mouse Plasma (Pubmed).

6. Pifferi, F et al (2011). Mild experimental ketosis increases brain uptake of 11C-acetoacetate and 18F-fluorodeoxyglucose: a dual-tracer PET imaging study in rats. Nutr Neurosci 14(2):51-8. Assay: Glycerol in Rat Plasma (Pubmed).

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1. Lung, M et al (2009). Diacylglycerol kinase epsilon is selective for both acyl chains of phosphatidic acid or diacylglycerol. J Biol Chem 284(45):31062-73. Assay: Glycerol in N/A Diacylglycerol (Pubmed).

2. Drew, BG et al (2011). Reconstituted high-density lipoprotein infusion modulates fatty acid metabolism in patients with type 2 diabetes mellitus. J Lipid Res 52(3):572-581. Assay: Glycerol in Human Plasma (Pubmed).

3. Wachtler, B et al (2011). From attachment to damage: defined genes of Candida albicans mediate adhesion, invasion and damage during interaction with oral epithelial cells. PLoS One 6(2):e17046. Assay: Glycerol in Fungi C. albicans (Pubmed).

4. Bahar, B et al. A potential role of IL-6 in the chito-oligosaccharide-mediated inhibition of adipogenesis (2011). Br J Nutr. 106(8):1142-1153. Assay: Glycerol in Mouse 3T3-L1 cells (Pubmed).

5. . Assay: Glycerol in Mouse Plasma (Pubmed).

6. Pifferi, F et al (2011). Mild experimental ketosis increases brain uptake of 11C-acetoacetate and 18F-fluorodeoxyglucose: a dual-tracer PET imaging study in rats. Nutr Neurosci 14(2):51-8. Assay: Glycerol in Rat Plasma (Pubmed).

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1. Lung, M et al (2009). Diacylglycerol kinase epsilon is selective for both acyl chains of phosphatidic acid or diacylglycerol. J Biol Chem 284(45):31062-73. Assay: Glycerol in N/A Diacylglycerol (Pubmed).

2. Drew, BG et al (2011). Reconstituted high-density lipoprotein infusion modulates fatty acid metabolism in patients with type 2 diabetes mellitus. J Lipid Res 52(3):572-581. Assay: Glycerol in Human Plasma (Pubmed).

3. Wachtler, B et al (2011). From attachment to damage: defined genes of Candida albicans mediate adhesion, invasion and damage during interaction with oral epithelial cells. PLoS One 6(2):e17046. Assay: Glycerol in Fungi C. albicans (Pubmed).

4. Bahar, B et al. A potential role of IL-6 in the chito-oligosaccharide-mediated inhibition of adipogenesis (2011). Br J Nutr. 106(8):1142-1153. Assay: Glycerol in Mouse 3T3-L1 cells (Pubmed).

5. . Assay: Glycerol in Mouse Plasma (Pubmed).

6. Pifferi, F et al (2011). Mild experimental ketosis increases brain uptake of 11C-acetoacetate and 18F-fluorodeoxyglucose: a dual-tracer PET imaging study in rats. Nutr Neurosci 14(2):51-8. Assay: Glycerol in Rat Plasma (Pubmed).

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