EnzyChrom™ Glycerol Assay Kit

• Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 10 to 1000 μM (92 μg/dL to 9.2 mg/dL) glycerol for colorimetric assays and 2 to 50 μM for fluorimetric assays.

• Simple and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature, compatible for HTS assays. 

• Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. 

Glycerol [Glycerin or Glycerine, C3H5 (OH) 3] is widely used in foods, beverages and pharmaceutical formulations. It is also a main by-product of biodiesel production. Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide applications. BioAssay Systems’ glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to glycerol concentration in the sample.