EnzyChrom™ HDL and LDL/VLDL Assay KitEnzyChrom™ HDL and LDL/VLDL Assay Kit
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EnzyChrom™ HDL and LDL/VLDL Assay Kit

For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.

• Sensitive and accurate. Requires only 20 μL serum sample. Detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay.

• Convenient. Room temperature assay. No 37°C heater is needed. 

Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors. Simple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable. BioAssay Systems’ HDL and LDL/VLDL quantification kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using cholesterol esterase/cholesterol dehydrogenase reagent. In this reaction, NAD is reduced to NADH. The optical density of the formed NADH at 340 nm is directly proportionate to the cholesterol concentration in the sample.

Cat# Size Price Qty Buy
EHDL-100 100 Tests £369.15

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.
Method OD340nm
Samples Serum
Species All
Detection Limit 5 mg/dL
Storage -20°C
Shelf Life 6 months
References Assay: HDL/LDL in mice plasma (Pubmed).

2. Uddin MJ et al (2011) Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genet 12:62. Assay: HDL/LDL in pig serum (Pubmed).

3. Labib, HM et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: HDL/LDL in Mouse Blood (Pubmed).

4. Ponda, MP et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis 210 (1): 57-62. Assay: HDL/LDL in Human Plasma (Pubmed).

5. Shanmugasundaram R & Selvaraj RK (2011) Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis. J Anim Physiol Anim Nutr(Berl) 95(6):762-70. Assay: HDL/LDL in Mouse blood (Pubmed).

6. Shon, SM et al (2011). Exercise attenuates matrix metalloproteinase activity in preexisting atherosclerotic plaque. Atherosclerosis 216 (1): 67-73. Assay: HDL/LDL in Rat Serum (Pubmed).

7. Oliver SR et al (2010). Increased oxidative stress and altered substrate metabolism in obese children. Int J Pediatr Obes. 2010 Oct;5(5):436-44. Assay: HDL/LDL in Bird serum (Pubmed).

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1. Bourdon JA et al (2012) Hepatic and pulmonary toxicogenomic profiles in mice intratracheally instilled with carbon black nanoparticles reveal pulmonary inflammation, acute phase response, and alterations in lipid homeostasis. Toxicol Sci 127(2):474-84. Assay: HDL/LDL in mice plasma (Pubmed).

2. Uddin MJ et al (2011) Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genet 12:62. Assay: HDL/LDL in pig serum (Pubmed).

3. Labib, HM et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: HDL/LDL in Mouse Blood (Pubmed).

4. Ponda, MP et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis 210 (1): 57-62. Assay: HDL/LDL in Human Plasma (Pubmed).

5. Shanmugasundaram R & Selvaraj RK (2011) Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis. J Anim Physiol Anim Nutr(Berl) 95(6):762-70. Assay: HDL/LDL in Mouse blood (Pubmed).

6. Shon, SM et al (2011). Exercise attenuates matrix metalloproteinase activity in preexisting atherosclerotic plaque. Atherosclerosis 216 (1): 67-73. Assay: HDL/LDL in Rat Serum (Pubmed).

7. Oliver SR et al (2010). Increased oxidative stress and altered substrate metabolism in obese children. Int J Pediatr Obes. 2010 Oct;5(5):436-44. Assay: HDL/LDL in Bird serum (Pubmed).

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1. Bourdon JA et al (2012) Hepatic and pulmonary toxicogenomic profiles in mice intratracheally instilled with carbon black nanoparticles reveal pulmonary inflammation, acute phase response, and alterations in lipid homeostasis. Toxicol Sci 127(2):474-84. Assay: HDL/LDL in mice plasma (Pubmed).

2. Uddin MJ et al (2011) Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genet 12:62. Assay: HDL/LDL in pig serum (Pubmed).

3. Labib, HM et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: HDL/LDL in Mouse Blood (Pubmed).

4. Ponda, MP et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis 210 (1): 57-62. Assay: HDL/LDL in Human Plasma (Pubmed).

5. Shanmugasundaram R & Selvaraj RK (2011) Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis. J Anim Physiol Anim Nutr(Berl) 95(6):762-70. Assay: HDL/LDL in Mouse blood (Pubmed).

6. Shon, SM et al (2011). Exercise attenuates matrix metalloproteinase activity in preexisting atherosclerotic plaque. Atherosclerosis 216 (1): 67-73. Assay: HDL/LDL in Rat Serum (Pubmed).

7. Oliver SR et al (2010). Increased oxidative stress and altered substrate metabolism in obese children. Int J Pediatr Obes. 2010 Oct;5(5):436-44. Assay: HDL/LDL in Bird serum (Pubmed).

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