EnzyChrom™ Isocitrate Assay KitEnzyChrom™ Isocitrate Assay Kit
Move your mouse over image or click to enlarge

EnzyChrom™ Isocitrate Assay Kit

For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.

• Fast and sensitive. Linear detection range (20 μL sample): 20 to 5000 μM for 10 min reaction. 

• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. BioAssay Systems’ isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the isocitrate concentration.

Cat# Size Price Qty Buy
ECIC-100 100 Tests £309.65

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.
Method OD565nm
Samples Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.) 
Species All
Detection Limit 20 μM
Storage -20°C
Shelf Life 6 months

Related Documents