EnzyChrom™ Lactose Assay KitEnzyChrom™ Lactose Assay Kit
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EnzyChrom™ Lactose Assay Kit

For quantitative determination of lactose and evaluation of drug effects on lactose metabolism.

• Use as little as 20 μL samples. Linear detection range in 96-well plate: 17 to 2000 μM lactose for colorimetric assays and 6 to 100 μM for fluorimetric assays.

Lactose (C12H22O11), also called milk sugar, is a disaccharide that consists of β-D-galactose and α/β-D-glucose through a β1-4 glycosidic linkage. Lactose is the major sugar and makes up 2–8% of milk. Simple, direct and high-throughput assays for lactose determination find wide applications. BioAssay Systems’ assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample.

Cat# Size Price Qty Buy
ELAC-100 100 Tests £318.15

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of lactose and evaluation of drug effects on lactose metabolism.
Method OD570nm, or FL530/585nm
Samples Biological (milk), food and agriculture etc
Species All
Detection Limit 6 μM
Storage -20°C
Shelf Life 12 months
References Assay: Milk in Rats (Pubmed).

2. Vabbilisetty, P and S Xue-Long (2014). Liposome surface functionalization based on different anchoring lipids via Staudinger ligation. Organic and Biomolecular Chemistry 12(8): 1237-44.Assay: Liposomes (Pubmed).

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1. Kwon, DH et al (2012). Dietary protein restriction induces steatohepatitis and alters leptin/signal transducers and activators of transcription 3 signaling in lactating rats. Journal of Nutritional Biochemistry 23(7): 791-9. Assay: Milk in Rats (Pubmed).

2. Vabbilisetty, P and S Xue-Long (2014). Liposome surface functionalization based on different anchoring lipids via Staudinger ligation. Organic and Biomolecular Chemistry 12(8): 1237-44.Assay: Liposomes (Pubmed).

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1. Kwon, DH et al (2012). Dietary protein restriction induces steatohepatitis and alters leptin/signal transducers and activators of transcription 3 signaling in lactating rats. Journal of Nutritional Biochemistry 23(7): 791-9. Assay: Milk in Rats (Pubmed).

2. Vabbilisetty, P and S Xue-Long (2014). Liposome surface functionalization based on different anchoring lipids via Staudinger ligation. Organic and Biomolecular Chemistry 12(8): 1237-44.Assay: Liposomes (Pubmed).

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