EnzyChrom™ Phospholipd Assay KitEnzyChrom™ Phospholipd Assay Kit
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EnzyChrom™ Phospholipd Assay Kit

For quantitative determination of phospholipid and evaluation of drug effects on its metabolism.

• Sensitive. Use 20 μL samples. Linear detection range: colorimetric assay 3 - 200 μM, fluorimetric assay 0.6 - 20 μM phospholipid.

Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. BioAssay Systems’ method provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.

Cat# Size Price Qty Buy
EPLP-100 100 Tests £335.15

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of phospholipid and evaluation of drug effects on its metabolism.
Method OD570nm, or FL530/585nm
Samples Biological
Species All
Detection Limit OD, FL: 3, 0.6 μM
Storage -20°C
Shelf Life 6 months
References Assay: Phospholipid in Human Red blood cell (Pubmed).

2. Kim S et al (2012) Aberrant upregulation of astroglial ceramide potentiates oligodendrocyte injury. Brain Pathol 22(1):41-47. Assay: Phospholipid in N/A lipid (Pubmed).

3. Soga N et al (2012) Kinetic equivalence of transmembrane pH and electrical potential differences in ATP synthesis. J Biol Chem 287(12):9633-9. Assay: Phospholipid in human cancer cell (Pubmed).

4. Haynes TA et al (2012) DNA damage induces down-regulation of UDP-glucose ceramide glucosyltransferase, increases ceramide levels and triggers apoptosis in p53-deficient cancer cells. Biochim Biophys Acta 1821(7):943-53. Assay: Phospholipid in Human Ovarian cancer cells (Pubmed).

5. Stoll C et al (2011). Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochim Biophys Acta 1808(1):474-481. Assay: Phospholipid in Mouse Brain (Pubmed).

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1. Methods for treatment of a sarcoma using an epimetabolic shifter (Coenzyme q10. US 2011/0064747. Assay: Phospholipid in Human Red blood cell (Pubmed).

2. Kim S et al (2012) Aberrant upregulation of astroglial ceramide potentiates oligodendrocyte injury. Brain Pathol 22(1):41-47. Assay: Phospholipid in N/A lipid (Pubmed).

3. Soga N et al (2012) Kinetic equivalence of transmembrane pH and electrical potential differences in ATP synthesis. J Biol Chem 287(12):9633-9. Assay: Phospholipid in human cancer cell (Pubmed).

4. Haynes TA et al (2012) DNA damage induces down-regulation of UDP-glucose ceramide glucosyltransferase, increases ceramide levels and triggers apoptosis in p53-deficient cancer cells. Biochim Biophys Acta 1821(7):943-53. Assay: Phospholipid in Human Ovarian cancer cells (Pubmed).

5. Stoll C et al (2011). Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochim Biophys Acta 1808(1):474-481. Assay: Phospholipid in Mouse Brain (Pubmed).

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1. Methods for treatment of a sarcoma using an epimetabolic shifter (Coenzyme q10. US 2011/0064747. Assay: Phospholipid in Human Red blood cell (Pubmed).

2. Kim S et al (2012) Aberrant upregulation of astroglial ceramide potentiates oligodendrocyte injury. Brain Pathol 22(1):41-47. Assay: Phospholipid in N/A lipid (Pubmed).

3. Soga N et al (2012) Kinetic equivalence of transmembrane pH and electrical potential differences in ATP synthesis. J Biol Chem 287(12):9633-9. Assay: Phospholipid in human cancer cell (Pubmed).

4. Haynes TA et al (2012) DNA damage induces down-regulation of UDP-glucose ceramide glucosyltransferase, increases ceramide levels and triggers apoptosis in p53-deficient cancer cells. Biochim Biophys Acta 1821(7):943-53. Assay: Phospholipid in Human Ovarian cancer cells (Pubmed).

5. Stoll C et al (2011). Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochim Biophys Acta 1808(1):474-481. Assay: Phospholipid in Mouse Brain (Pubmed).

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