Diabetes/Obesity

For quantitatve determination of cholesterol uptake in cells and screening of modulators on cholesterol uptake. Key Features: Convenient. Treat cells directly in 96-well fluorescent plate. Safe. Non-radioactive assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day Method: FL485/535nm. Samples: Adherent cells. Species: All. Procedure: Assay takes 24-72 hrs, hand-on time 1 hr. Kit size: 100 tests.

For quantitatve determination of β-N-Acetylglucosaminidase activity and evaluation of drug modulators. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.2 to 50 U/L for a 30 minute reaction at 37°C. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD405nm. Samples: Urine, serum, plasma, cell lysate, etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.2 U/L.

For quantitative determination of α-L-Fucosidase activity in biological samples. Key Features: High sensitivity and wide linear range. Linear detection range (10 µL sample): 1 to 100 U/L. Homogeneous and simple procedure. Simple add-mix-read procedure allows reliable quantitation of fucosidase activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Can be readily automated to measure thousands of samples per day. Method: OD405nm. Samples: Plasma, serum, tissue, cell lysate, etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 1 U/L.

For quantitative determination of uric acid and evaluation of drug effects on uric acid metabolism. Key Features: Sensitive and accurate. Use 5 µL samples. Linear detection range 0.22 mg/dL (13 µM) to 30 mg/dL (1785 µM) uric acid in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples. Method: OD590nm. Samples: Serum, plasma, urine and other biological samples. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.2 mg/dL (13 µM).

For quantitative enzymatic determination of urea. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 1 mg/mL (0.17 mM) to 100 mg/mL (17 mM) urea in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 5 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay.Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD557nm. Samples: Urea in biological samples (e.g. plasma, serum, urine) and food/beverage samples (e.g. milk). Species: All. Procedure: Assay takes 5 min. Kit size: 100 tests. Detection limit: 1 mg/mL (0.17 mM).

For quantitative determination of urea and evaluation of drug effects on urea metabolism. Key Features: Sensitive and accurate. Use 5 µL samples. Linear detection range 0.08 mg/dL (13 µM) to 100 mg/dL (17 mM) urea in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein. Method: OD520nm (Chemical). Samples: Serum, plasma, urine, milk, cell/tissue culture, bronchoalveolar lavage (BAL), food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.08 mg/dL (13 µM).

For quantitative determination of sorbitol dehydrogenase enzyme activity. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.1 to 125 U/L for 12 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples e.g. plasma, serum, urine, tissue and culture media. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.1 U/L.

For quantitative determination of lipase enzyme activity. Key Features: Sensitive and accurate. Linear detection range 40 to 1600 U/L lipase activity in 96-well plate assay. Convenient and high throughput. The procedure involves adding a single working reagent, and reading the optical density at 10 min and 20 min at room temperature or 37°C. Can be automated to process thousands of samples per day. Method: OD412nm. Samples: Serum, plasma, saliva, urine etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 40 U/L.

For quantitative colorimetric determination of glucose-6-phosphate concentration and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.2 to 100 U/L for 15 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD460nm. Samples: Plasma, serum, tissue and culture media, etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.2 U/L.

For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 200 U/L for 15 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. plasma, serum, tissue and culture media). Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.5 U/L.

For quantitative determination of long-chain fatty acid uptake in whole cells and evaluation of effects of ligands or drugs on fatty acid transport. Key Features: Safe. Non-radioactive assay. Fast and Sensitive. Homogenous "add-and-read" assay. No wash, lysis, or staining steps needed. Simple and Convenient. Can be automated as a high-throughput assay for fatty acid transport and modulator screens in cells. Method: FL 488/523nm. Samples: Adipocytes and other fatty acid transporting cells. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 Tests.

For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. Key Features: Sensitive and accurate. Detection limit of 0.02 µM and linearity up to 1 µM NAD+/NADH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: F530/585nm. Samples: Cell, tissue extracts etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.02 µM.

For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 1 µM and linearity up to 50 µM L-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585 nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 1 µM.

For quantitative fluorimetric determination of isocitrate and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.6 to 500 µM for 10 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585 nm. Samples: Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.). Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.6 µM.

For determination of RPS6(S235/236) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, rat and mouse. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

For determination of RB(T821/826) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, rat and mouse. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

For determination of LKB1(S431) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, mouse, and rat. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

For determination of GSK3B(S9) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, rat and mouse. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

For determination of GSK3A(S21) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, rat, mouse, and canine. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

For determination of AKT1(S473) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human, rat and mouse. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

For determination of 4EBP1(S65) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human (S65), mouse/rat (S64). Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

For determination of SHP2(Y542) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-Based ELISA (FL530/585 nm, 360/450 nm). Samples: Cells. Species: human. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

For quantitative determination of glucose uptake in whole cells and evaluation of effects of ligands or drugs on glucose transport. Key Features: Safe. No radioactive material is used. Sensitive and Accurate. Detection limit of 0.1 µM and linearity up to 5 µM 2-DG6P. Simple and Convenient. Can be automated as a medium throughput assay for glucose transport in cells. Method: FL530/585nm. Samples: Cell culture. Species: Mammalian. Procedure: Assay takes Approximately 2 hrs. Kit size: 100 tests. Detection limit: 0.1 µM.

For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 1 µM and linearity up to 50 µM D-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585 nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 1 µM.

For quantitative enzymatic determination of urea. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 50 to 1000 µM urea in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay.Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD340nm. Samples: Urea in biological samples (e.g. plasma, serum, urine, bronchoalveolar lavage (BAL)) and food/beverage samples (e.g. milk). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 50 µM.

For quantitative determination of triglyceride and evaluation of drug effects on triglyceride metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range 0.01 to 1.0 mmol/L (0.88 to 88.5 mg/dL) triglyceride. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Method: OD570nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 30 min. Kit size: 200 tests. Detection limit: 0.01 mmol/L (0.88 mg/dL).

For quantitative determination of succinate (succinic acid) in food, beverage, agricultural products, and other biological samples. Key Features: Fast and sensitive. Use 20 µL sample. Linear detection range 10 to 400 µM for colorimetric assays and 2 to 40 µM for fluorimetric assays. Convenient. The procedure involves adding a single working reagent, and reading the absorbance or fluorescence after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD570nm, FL530/585nm. Samples: succinate in food, beverage, agricultural products, and other biological samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: Colorimetric assay: 10 µM; Fluorimetric assay: 2 µM.

For quantitative determination of D-sorbitol and evaluation of drug effects on sorbitol metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 5 to 1000 µM D-sorbitol. Convenient and high-throughput. Room temperature assay. No 37°C incubator required. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological (e.g. blood), food, beverage and agriculture samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 5 µM.

For quantitative determination of pyruvate kinase (PK) activity and evaluation of drug effects on PK activity. Key Features: Sensitive and accurate. Linear detection range in 96-well plate: 0.1 to 50 U/L for colorimetric assays and 0.01 to 2 U/L for fluorimetric assays run at 25°C for 30 min. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, FL530/590nm. Samples: Plasma, serum, cell and tissue etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.01 U/L.

For quantitative determination of pyruvate and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 2 to 500 µM (17 µg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 µM for fluorimetric assays. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 2, 0.2 µM.

For quantitative determination of oxalate (oxalic acid) in urine and other samples. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 10 minute incubation: 20 to 1500 µM oxalate. Fast and convenient. Sample pre-treatment is faster and easier than using activated carbon in competitor’s assay kits. High-throughput adaptable. The procedure involves addition of a single working reagent and incubation for 10 min at room temperature. Can be automated for processing thousands of samples per day. Method: OD595nm. Samples: urine, animal and plant tissue samples. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 20 µM.

For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 µM and linearity up to 10 µM NAD+/NADH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 15 min at room temperature. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Cell or tissue extracts. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.05 µM.

For quantitative determination of maltose and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 10 µL sample. Linear detection range 2 to 500 µM maltose for colorimetric assays and 1 to 50 µM for flourimetric assays. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, urine, food and beverages, etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 2 µM.

For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 65 U/L for 20 min reaction at 37°C. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 U/L.

For quantitative determination of L-malate (L-malic acid) and drug effects on malate metabolism. Key Features: Fast and sensitive. Use of 20 µL sample. Linear detection range 0.02 to 2 mM L-malate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time 15 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Malate in food, juice, beverage and other agricultural products. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.02 mM.

For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.05 mM.

For quantitative determination of ketone body (acetoacetate and 3-hydroxybutyrate) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Uses 10 µL sample. Linear detection range of 0.12 to 8 mM for each ketone body in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at room temperature. High-throughput. Can be automated as a high-throughput 96-well plate assay for many samples per day. Method: OD340nm. Samples: Serum, plasma, urine etc. Species: All. Procedure: Assay takes 30 min. Kit size: 200 tests. Detection limit: 0.12 mM.

For quantitative colorimetric determination of isocitrate and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 20 to 5000 µM for 10 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.). Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 20 µM.

For quantitative determination of glycogen and evaluation of drug effects on glycogen metabolism. Key Features: Use as little as 10 µL samples. Linear detection range: 2 to 200 µg/mL glycogen for colorimetric assays and 0.2 to 20 µg/mL for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: OD, FL: 2, 0.2 µg/mL.

For quantitative determination of glucose-6-phosphate in biological samples. Key Features: Fast and sensitive. Linear detection range: 10 to 1000 µM G6P. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD460nm. Samples: Plasma, serum, tissue and culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 10 µM.

For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate: 0.03 to 2 mM (0.54 mg/dL to 36 mg/dL) glucose. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, urine, saliva, milk, culture medium, food, beverages etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.03 mM.

For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate: 0.1 to 3 mM (1.8 mg/dL to 54 mg/dL) glucose for colorimetric assays. Convenient. Room temperature assay. No 37°C heater is needed. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD340nm. Samples: Serum, plasma, urine, saliva, milk, culture medium, food, beverages etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.1 mM.

For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate: 5 to 300 µM (90 µg/dL to 5.4 mg/dL) glucose for colorimetric assays and 1 to 30 µM for fluorimetric assays. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, milk, culture medium, food, agriculture etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 5 µM.

For quantitative determination of fumarate and drug effects on its metabolism. Key Features: Fast and sensitive. Use of 20 µL sample. Linear detection range 0.005 to 2 mM fumarate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Food, beverage and other biological samples (e.g. cell lysate, tissue homogenate, serum). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 5 µM.

For quantitative determination of fumarase enzyme activity. Key Features: Fast and sensitive. Linear detection range: 0.4 to 70 U/L for 30 min reaction at 37°C. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media). Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.4 U/L.

For quantitative determination of fatty acid and evaluation of drug effects on its metabolism. Key Features: Sensitive. Use 10 µL samples. Linear detection range: colorimetric assay 7 - 1000 µM, fluorimetric assay 7 - 100 µM fatty acid. Convenient. Room temperature "mix-and-read" procedure can be readily automated for high-throughput assay of thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, milk, cell cultures, food, agriculture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 7 µM.

For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565 nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.05 mM.

For quantitative determination of creatine kinase (CK) activity and evaluation of drug effects on CK activity. Key Features: Sensitive and accurate. Detection range: 5 to 300 U/L creatine kinase in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at 20 min and 40 min at room temperature or 37°C. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD340nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 5 U/L.

For quantitative determination of coenzyme A (CoA) and evaluation of drug effects on CoA metabolism. Key Features: Sensitive. Use 10 µL samples. Linear detection range: colorimetric assay 5 - 1000 µM, fluorimetric assay 3 - 100 µM CoA. Convenient. Room temperature "mix-and-read" procedure can be readily automated for high-throughput assay of thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 3 µM.