EnzyChrom™ Ascorbic Acid Assay KitEnzyChrom™ Ascorbic Acid Assay Kit
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EnzyChrom™ Ascorbic Acid Assay Kit

For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism.

• Use 20 μL samples. Linear detection range: colorimetric assay 6 to 1000 μM, fluorimetric assay 1 to 100 μM ascorbic acid.

Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages. BioAssay Systems’ method provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample.

Cat# Size Price Qty Buy
EASC-100 100 Tests £318.15

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism.
Method OD570nm, or FL530/585nm
Samples Serum, plasma, urine, saliva, milk, tissue, and cell culture
Species All
Detection Limit OD, FL: 6, 1 μM
Storage -20°C
Shelf Life 6 months
References Assay: Plasma in Humans (Pubmed).

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1. Trezzi, JP et al (2016). LacaScore: a novel plasma sample quality control tool based on ascorbic acid and lactic acid levels. Metabolomics 12: 96. Assay: Plasma in Humans (Pubmed).

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1. Trezzi, JP et al (2016). LacaScore: a novel plasma sample quality control tool based on ascorbic acid and lactic acid levels. Metabolomics 12: 96. Assay: Plasma in Humans (Pubmed).

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