EnzyChrom™ Creatine Kinase Assay KitEnzyChrom™ Creatine Kinase Assay Kit
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EnzyChrom™ Creatine Kinase Assay Kit

For quantitative determination of creatine kinase activity and evaluation of drug effects on CK activity.

• Sensitive and accurate. Detection range: 5 to 300 U/L creatine kinase in 96-well plate assay.

• Convenient. The procedure involves adding a single working reagent, and reading the optical density at 20 min and 40 min at room temperature or 37°C. 

• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

CREATINE KINASE (CK), also known as creatine phosphokinase (CPK), is an enzyme (EC 2.7.3.2) expressed predominantly in skeletal muscle, smooth muscle and the brain. The CK enzyme consists of two subunits, which can be either B (brain type) or M (muscle type), and hence three different isoenzymes: CK-MM, CK-BB and CK-MB. CK catalyzes the conversion of creatine to phosphocreatine, consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP) and the reverse reaction. CK is often determined routinely in emergency patients with chest pain and acute renal failure. Elevation of CK is an indication of damage to muscle and has been associated with injury, rhabdomyolysis, myocardial infarction, myositis, myocarditis, malignant hyperthermia and neuroleptic malignant syndrome, etc. Lower levels can be an indication of alcoholic liver disease and rheumatoid arthritis. Simple, direct and automation-ready procedures for measuring CK activity are very desirable. BioAssay Systems’ EnzyChrom™ Creatine Kinase Assay Kit is based on enzyme coupled reactions in which creatine phosphate and ADP is converted to creatine and ATP by CK, the generated ATP is used to phosphorylate glucose by hexokinase to generate glucose-6-phosphate, which is then oxidized by NADP in the presence of glucose-6-phosphate dehydrogenase. The produced NADPH, measured at 340 nm, is proportionate to the CK activity in the sample.

Cat# Size Price Qty Buy
ECPK-100 100 Tests £386.15

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of creatine kinase activity and evaluation of drug effects on CK activity.
Method OD340nm
Samples Serum, plasma etc
Species All
Detection Limit 5 U/L
Storage -20°C
Shelf Life 6 months
References Assay: Creatine Kinase in Rat Serum (Pubmed).

2. Branciari, R et al (2009). Effect of genotype and rearing system on chicken behavior and muscle fiber characteristics. J Anim Sci 87(12):4109-17. Assay: Creatine Kinase in Chicken Serum (Pubmed).

3. Annecke, T et al (2010). Sevoflurane preserves the endothelial glycocalyx against ischaemia-reperfusion injury. Br J Anaesth 104(4):414-21. Assay: Creatine Kinase in Guinea Pig Heart transudate (Pubmed).

4. Kwon, S et al (2010). ASB9 interacts with ubiquitous mitochondrial creatine kinase and inhibits mitochondrial function. BMC Biol 8:23. Assay: Creatine Kinase in Human HEK 293 cells (Pubmed).

5. Steen, C et al (2010). Reduced creatine kinase B activity in multiple sclerosis normal appearing white matter. PLoS One 5(5):e10811. Assay: Creatine Kinase in Human Brain tissue (Pubmed).

6. Pruksakorn, D et al (2010). Metabolic alteration of HepG2 in scaffold-based 3-D culture: proteomic approach. Proteomics 10(21):3896-904. Assay: Creatine Kinase in Human HepG2 hepatocellular carcinoma cells (Pubmed).

7. Lu, X et al (2009). Activation of NF-kappaB is a critical element in the antiapoptotic effect of anesthetic preconditioning. Am J Physiol Heart Circ Physiol 296(5):H1296-304. Assay: Creatine Kinase in Rat Heart perfusate (Pubmed).

8. Itaka, K et al (2010). Polyplex nanomicelle promotes hydrodynamic gene introduction to skeletal muscle. J Control Release 143(1):112-9. Assay: Creatine Kinase in Mouse Serum (Pubmed).

9. Shi, H et al (2010). MAP kinase phosphatase-1 deficiency impairs skeletal muscle regeneration and exacerbates muscular dystrophy. FASEB J 24(8):2985-97. Assay: Creatine Kinase in Mouse Serum (Pubmed).

10. Gawlik KI, Durbeej M (2010). Transgenic overexpression of laminin alpha1 chain in laminin alpha2 chain-deficient mice rescues the disease throughout the lifespan. Muscle Nerve 42(1):30-7. Assay: Creatine Kinase in Mouse Serum (Pubmed).

11. Lu, X et al (2011). Phosphorylation of ARC is a critical element in the antiapoptotic effect of anesthetic preconditioning. Anesth Analg 112(3):525-31. Assay: Creatine Kinase in Rat Heart perfusate (Pubmed).

12. Li, S et al (2011). Reduction of cold ischemia-reperfusion injury by graft-expressing clusterin in heart transplantation. J Heart Lung Transplant 30(7):819-26. Assay: Creatine Kinase in Mouse Heart preservation solution (Pubmed).

13. Ren, JY et al (2011). Cardioprotection by ischemic postconditioning is lost in isolated perfused heart from diabetic rats: Involvement of transient receptor potential vanilloid 1, calcitonin gene-related peptide and substance P. Regul Pept 169(1-3):49-57. Assay: Creatine Kinase in Rat Heart perfusate (Pubmed).

14. Uchida, S et al (2011). Combination of chondroitin sulfate and polyplex micelles from Poly(ethylene glycol)-poly{N’-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} block copolymer for prolonged in vivo gene transfection with reduced toxicity. J Control Release . Assay: Creatine Kinase in Mouse Serum (Pubmed).

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1. Kim, YM et al (2009). Palmatine from Coptidis rhizoma reduces ischemia-reperfusion-mediated acute myocardial injury in the rat. Food Chem Toxicol 47(8):2097-102. Assay: Creatine Kinase in Rat Serum (Pubmed).

2. Branciari, R et al (2009). Effect of genotype and rearing system on chicken behavior and muscle fiber characteristics. J Anim Sci 87(12):4109-17. Assay: Creatine Kinase in Chicken Serum (Pubmed).

3. Annecke, T et al (2010). Sevoflurane preserves the endothelial glycocalyx against ischaemia-reperfusion injury. Br J Anaesth 104(4):414-21. Assay: Creatine Kinase in Guinea Pig Heart transudate (Pubmed).

4. Kwon, S et al (2010). ASB9 interacts with ubiquitous mitochondrial creatine kinase and inhibits mitochondrial function. BMC Biol 8:23. Assay: Creatine Kinase in Human HEK 293 cells (Pubmed).

5. Steen, C et al (2010). Reduced creatine kinase B activity in multiple sclerosis normal appearing white matter. PLoS One 5(5):e10811. Assay: Creatine Kinase in Human Brain tissue (Pubmed).

6. Pruksakorn, D et al (2010). Metabolic alteration of HepG2 in scaffold-based 3-D culture: proteomic approach. Proteomics 10(21):3896-904. Assay: Creatine Kinase in Human HepG2 hepatocellular carcinoma cells (Pubmed).

7. Lu, X et al (2009). Activation of NF-kappaB is a critical element in the antiapoptotic effect of anesthetic preconditioning. Am J Physiol Heart Circ Physiol 296(5):H1296-304. Assay: Creatine Kinase in Rat Heart perfusate (Pubmed).

8. Itaka, K et al (2010). Polyplex nanomicelle promotes hydrodynamic gene introduction to skeletal muscle. J Control Release 143(1):112-9. Assay: Creatine Kinase in Mouse Serum (Pubmed).

9. Shi, H et al (2010). MAP kinase phosphatase-1 deficiency impairs skeletal muscle regeneration and exacerbates muscular dystrophy. FASEB J 24(8):2985-97. Assay: Creatine Kinase in Mouse Serum (Pubmed).

10. Gawlik KI, Durbeej M (2010). Transgenic overexpression of laminin alpha1 chain in laminin alpha2 chain-deficient mice rescues the disease throughout the lifespan. Muscle Nerve 42(1):30-7. Assay: Creatine Kinase in Mouse Serum (Pubmed).

11. Lu, X et al (2011). Phosphorylation of ARC is a critical element in the antiapoptotic effect of anesthetic preconditioning. Anesth Analg 112(3):525-31. Assay: Creatine Kinase in Rat Heart perfusate (Pubmed).

12. Li, S et al (2011). Reduction of cold ischemia-reperfusion injury by graft-expressing clusterin in heart transplantation. J Heart Lung Transplant 30(7):819-26. Assay: Creatine Kinase in Mouse Heart preservation solution (Pubmed).

13. Ren, JY et al (2011). Cardioprotection by ischemic postconditioning is lost in isolated perfused heart from diabetic rats: Involvement of transient receptor potential vanilloid 1, calcitonin gene-related peptide and substance P. Regul Pept 169(1-3):49-57. Assay: Creatine Kinase in Rat Heart perfusate (Pubmed).

14. Uchida, S et al (2011). Combination of chondroitin sulfate and polyplex micelles from Poly(ethylene glycol)-poly{N’-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} block copolymer for prolonged in vivo gene transfection with reduced toxicity. J Control Release . Assay: Creatine Kinase in Mouse Serum (Pubmed).

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1. Kim, YM et al (2009). Palmatine from Coptidis rhizoma reduces ischemia-reperfusion-mediated acute myocardial injury in the rat. Food Chem Toxicol 47(8):2097-102. Assay: Creatine Kinase in Rat Serum (Pubmed).

2. Branciari, R et al (2009). Effect of genotype and rearing system on chicken behavior and muscle fiber characteristics. J Anim Sci 87(12):4109-17. Assay: Creatine Kinase in Chicken Serum (Pubmed).

3. Annecke, T et al (2010). Sevoflurane preserves the endothelial glycocalyx against ischaemia-reperfusion injury. Br J Anaesth 104(4):414-21. Assay: Creatine Kinase in Guinea Pig Heart transudate (Pubmed).

4. Kwon, S et al (2010). ASB9 interacts with ubiquitous mitochondrial creatine kinase and inhibits mitochondrial function. BMC Biol 8:23. Assay: Creatine Kinase in Human HEK 293 cells (Pubmed).

5. Steen, C et al (2010). Reduced creatine kinase B activity in multiple sclerosis normal appearing white matter. PLoS One 5(5):e10811. Assay: Creatine Kinase in Human Brain tissue (Pubmed).

6. Pruksakorn, D et al (2010). Metabolic alteration of HepG2 in scaffold-based 3-D culture: proteomic approach. Proteomics 10(21):3896-904. Assay: Creatine Kinase in Human HepG2 hepatocellular carcinoma cells (Pubmed).

7. Lu, X et al (2009). Activation of NF-kappaB is a critical element in the antiapoptotic effect of anesthetic preconditioning. Am J Physiol Heart Circ Physiol 296(5):H1296-304. Assay: Creatine Kinase in Rat Heart perfusate (Pubmed).

8. Itaka, K et al (2010). Polyplex nanomicelle promotes hydrodynamic gene introduction to skeletal muscle. J Control Release 143(1):112-9. Assay: Creatine Kinase in Mouse Serum (Pubmed).

9. Shi, H et al (2010). MAP kinase phosphatase-1 deficiency impairs skeletal muscle regeneration and exacerbates muscular dystrophy. FASEB J 24(8):2985-97. Assay: Creatine Kinase in Mouse Serum (Pubmed).

10. Gawlik KI, Durbeej M (2010). Transgenic overexpression of laminin alpha1 chain in laminin alpha2 chain-deficient mice rescues the disease throughout the lifespan. Muscle Nerve 42(1):30-7. Assay: Creatine Kinase in Mouse Serum (Pubmed).

11. Lu, X et al (2011). Phosphorylation of ARC is a critical element in the antiapoptotic effect of anesthetic preconditioning. Anesth Analg 112(3):525-31. Assay: Creatine Kinase in Rat Heart perfusate (Pubmed).

12. Li, S et al (2011). Reduction of cold ischemia-reperfusion injury by graft-expressing clusterin in heart transplantation. J Heart Lung Transplant 30(7):819-26. Assay: Creatine Kinase in Mouse Heart preservation solution (Pubmed).

13. Ren, JY et al (2011). Cardioprotection by ischemic postconditioning is lost in isolated perfused heart from diabetic rats: Involvement of transient receptor potential vanilloid 1, calcitonin gene-related peptide and substance P. Regul Pept 169(1-3):49-57. Assay: Creatine Kinase in Rat Heart perfusate (Pubmed).

14. Uchida, S et al (2011). Combination of chondroitin sulfate and polyplex micelles from Poly(ethylene glycol)-poly{N’-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} block copolymer for prolonged in vivo gene transfection with reduced toxicity. J Control Release . Assay: Creatine Kinase in Mouse Serum (Pubmed).

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