EnzyFluo™ Isocitrate Assay KitEnzyFluo™ Isocitrate Assay Kit
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EnzyFluo™ Isocitrate Assay Kit

For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.

• Fast and sensitive. Linear detection range (20 μL sample): 0.6 to 500 μM for 10 min reaction. 

• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. BioAssay Systems’ isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the isocitrate concentration in the sample.

Cat# Size Price Qty Buy
EFIC-100 100 Tests £309.65

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.
Method FL530/585 nm
Samples Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.) 
Species All
Detection Limit 0.6 μM
Storage -20°C
Shelf Life 6 months

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