QuantiChrom™ Glucose-6-Phosphate Dehydrogenase KitQuantiChrom™ Glucose-6-Phosphate Dehydrogenase Kit
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QuantiChrom™ Glucose-6-Phosphate Dehydrogenase Kit

For quantitative colorimetric determination of glucose-6-phosphate concentration and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.2 to 100 U/L for 15 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD460nm. Samples: Plasma, serum, tissue and culture media, etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.2 U/L.

• Fast and sensitive. Linear detection range (20 μL sample): 0.2 to 100 U/L for 15 min reaction. 

• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. BioAssay Systems’ non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

Cat# Size Price Qty Buy
DGPDH-100 100 Tests £373.15

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative colorimetric determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism.
Method OD460nm
Samples Plasma, serum, tissue and culture media, etc
Species All
Detection Limit 10 μM
Storage -20 °C
Shelf Life 15 months

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