QuantiChrom™ Uric Acid Assay KitQuantiChrom™ Uric Acid Assay Kit
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QuantiChrom™ Uric Acid Assay Kit

For quantitative determination of uric acid and evaluation of drug effects on uric acid metabolism.

• Sensitive and accurate. Use 5 μL samples. Linear detection range 0.22 mg/dL (13 μM) to 30 mg/dL (1785 μM) uric acid in 96-well plate assay.

• Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.

• Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.

• Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples.

Uric acid is the waste product produced from the degradation of purines. In healthy human, uric acid is filtered and removed from the blood by the kidneys and excreted into urine. Because a number of kidney diseases are known to affect uric acid levels, uric acid determination is thus important and useful in diagnosing and evaluating kidney diseases. For example, when uric acid is present in the blood at abnormally high levels, it tends to crystallize in body joints, resulting in gout, a very painful inflammatory condition. Increased levels of uric acid are also known to be associated with uremia, leukemia and pneumonia. Simple, direct and automation-ready procedures for measuring uric acid concentration in blood are becoming popular in Research and Drug Discovery. BioAssay Systems’ uric acid assay kit is designed to measure uric acid directly in serum without any pretreatment. The improved method utilizes 2,4,6-tripyridyl-s-triazine that forms a blue colored complex specifically with iron in the presence of uric acid. The intensity of the color, measured at 590nm, is directly proportional to the uric acid concentration in the serum. The optimized formulation substantially reduces interference by substances in the raw samples.

Cat# Size Price Qty Buy
DIUA-250 250 Tests £296.65

Additional Information

Property Value or Rating
Product Size 250 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of uric acid and evaluation of drug effects on uric acid metabolism.
Method OD590nm
Samples Serum, plasma, urine and other biological samples
Species All
Detection Limit 0.2 mg/dL (13 μM)
Storage 4, -20°C
Shelf Life 12 months
References Assay: Uric Acid in Mouse Peritoneal lavage fluid (Pubmed).

2. Thangaraju, M et al (2006). c/ebpdelta Null mouse as a model for the double knock-out of slc5a8 and slc5a12 in kidney. J Biol Chem 281(37):26769-73. Assay: Uric Acid in Mouse Serum, Urine (Pubmed).

3. Brosnahan, AJ et al (2009). Cytolysins augment superantigen penetration of stratified mucosa. J Immunol 182(4):2364-73. Assay: Uric Acid in Human Vaginal epithelial cells (Pubmed).

4. Yu, MA et al (2010). Oxidative stress with an activation of the renin-angiotensin system in human vascular endothelial cells as a novel mechanism of uric acid-induced endothelial dysfunction. J Hypertens 28(6):1234-42. Assay: Uric Acid in Human endothelial cell (Pubmed).

5. Xu, X et al (2011). Major histocompatibility complex class I-related chain A/B (MICA/B) expression in tumor tissue and serum of pancreatic cancer: role of uric acid accumulation in gemcitabine-induced MICA/B expression. BMC Cancer 11(1):194. Assay: Uric Acid in Human PANC-1 pancreatic cancer cell lysate (Pubmed).

6. Kaya, H et al (2008). The protective effect of N-acetylcysteine against cyclosporine A-induced hepatotoxicity in rats. J Appl Toxicol 28(1):15-20. Assay: Uric Acid in Rat Serum (Pubmed).

7. Kamel, AH (2007). Conventional and planar chip sensors for potentiometric assay of uric acid in biological fluids using flow injection analysis. J Pharm Biomed Anal 45(2):341-8. Assay: Uric Acid in Human Urine (Pubmed).

8. Whidden, MA et al (2009). Xanthine oxidase contributes to mechanical ventilation-induced diaphragmatic oxidative stress and contractile dysfunction. J Appl Physiol 106(2):385-94. Assay: Uric Acid in Rat Diaphragm (Pubmed).

9. Moon, HG et al (2010). Conversion of Th17-type into Th2-type inflammation by acetyl salicylic acid via the adenosine and uric acid pathway in the lung. Allergy 65(9):1093-103. Assay: Uric Acid in Mouse lung tissue (Pubmed).

10. Belarbi, M et al (2011). Oleaster Oil Positively Modulates Plasma Lipids in Humans. J Agric Food Chem . Assay: Uric Acid in Human Plasma (Pubmed).

11. Viel, EC et al (2008). Xanthine oxidase and mitochondria contribute to vascular superoxide anion generation in DOCA-salt hypertensive rats. Am J Physiol Heart Circ Physiol 295(1):H281-8. Assay: Uric Acid in Rat Plasma (Pubmed).

12. DiSilvestro RA, DiSilvestro DJ (2009). Pomegranate extract mouth rinsing effects on saliva measures relevant to gingivitis risk. Phytother Res 23(8):1123-7. Assay: Uric Acid in Human Saliva (Pubmed).

13. Karaouzene, N et al (2011). Effects of the association of aging and obesity on lipids, lipoproteins and oxidative stress biomarkers: A comparison of older with young men. Nutr Metab Cardiovasc Dis . Assay: Uric Acid in Human Plasma (Pubmed).

14. Smith, CL et al (2011). Naturally high plasma glucose levels in mourning doves (Zenaida macroura) do not lead to high levels of reactive oxygen species in the vasculature. Zoology (Jena) 114(3):171-6. Assay: Uric Acid in Bird Plasma (mourning dove) (Pubmed).

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1. Kono, H et al (2010). Uric acid promotes an acute inflammatory response to sterile cell death in mice. J Clin Invest 120(6):1939-49. Assay: Uric Acid in Mouse Peritoneal lavage fluid (Pubmed).

2. Thangaraju, M et al (2006). c/ebpdelta Null mouse as a model for the double knock-out of slc5a8 and slc5a12 in kidney. J Biol Chem 281(37):26769-73. Assay: Uric Acid in Mouse Serum, Urine (Pubmed).

3. Brosnahan, AJ et al (2009). Cytolysins augment superantigen penetration of stratified mucosa. J Immunol 182(4):2364-73. Assay: Uric Acid in Human Vaginal epithelial cells (Pubmed).

4. Yu, MA et al (2010). Oxidative stress with an activation of the renin-angiotensin system in human vascular endothelial cells as a novel mechanism of uric acid-induced endothelial dysfunction. J Hypertens 28(6):1234-42. Assay: Uric Acid in Human endothelial cell (Pubmed).

5. Xu, X et al (2011). Major histocompatibility complex class I-related chain A/B (MICA/B) expression in tumor tissue and serum of pancreatic cancer: role of uric acid accumulation in gemcitabine-induced MICA/B expression. BMC Cancer 11(1):194. Assay: Uric Acid in Human PANC-1 pancreatic cancer cell lysate (Pubmed).

6. Kaya, H et al (2008). The protective effect of N-acetylcysteine against cyclosporine A-induced hepatotoxicity in rats. J Appl Toxicol 28(1):15-20. Assay: Uric Acid in Rat Serum (Pubmed).

7. Kamel, AH (2007). Conventional and planar chip sensors for potentiometric assay of uric acid in biological fluids using flow injection analysis. J Pharm Biomed Anal 45(2):341-8. Assay: Uric Acid in Human Urine (Pubmed).

8. Whidden, MA et al (2009). Xanthine oxidase contributes to mechanical ventilation-induced diaphragmatic oxidative stress and contractile dysfunction. J Appl Physiol 106(2):385-94. Assay: Uric Acid in Rat Diaphragm (Pubmed).

9. Moon, HG et al (2010). Conversion of Th17-type into Th2-type inflammation by acetyl salicylic acid via the adenosine and uric acid pathway in the lung. Allergy 65(9):1093-103. Assay: Uric Acid in Mouse lung tissue (Pubmed).

10. Belarbi, M et al (2011). Oleaster Oil Positively Modulates Plasma Lipids in Humans. J Agric Food Chem . Assay: Uric Acid in Human Plasma (Pubmed).

11. Viel, EC et al (2008). Xanthine oxidase and mitochondria contribute to vascular superoxide anion generation in DOCA-salt hypertensive rats. Am J Physiol Heart Circ Physiol 295(1):H281-8. Assay: Uric Acid in Rat Plasma (Pubmed).

12. DiSilvestro RA, DiSilvestro DJ (2009). Pomegranate extract mouth rinsing effects on saliva measures relevant to gingivitis risk. Phytother Res 23(8):1123-7. Assay: Uric Acid in Human Saliva (Pubmed).

13. Karaouzene, N et al (2011). Effects of the association of aging and obesity on lipids, lipoproteins and oxidative stress biomarkers: A comparison of older with young men. Nutr Metab Cardiovasc Dis . Assay: Uric Acid in Human Plasma (Pubmed).

14. Smith, CL et al (2011). Naturally high plasma glucose levels in mourning doves (Zenaida macroura) do not lead to high levels of reactive oxygen species in the vasculature. Zoology (Jena) 114(3):171-6. Assay: Uric Acid in Bird Plasma (mourning dove) (Pubmed).

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1. Kono, H et al (2010). Uric acid promotes an acute inflammatory response to sterile cell death in mice. J Clin Invest 120(6):1939-49. Assay: Uric Acid in Mouse Peritoneal lavage fluid (Pubmed).

2. Thangaraju, M et al (2006). c/ebpdelta Null mouse as a model for the double knock-out of slc5a8 and slc5a12 in kidney. J Biol Chem 281(37):26769-73. Assay: Uric Acid in Mouse Serum, Urine (Pubmed).

3. Brosnahan, AJ et al (2009). Cytolysins augment superantigen penetration of stratified mucosa. J Immunol 182(4):2364-73. Assay: Uric Acid in Human Vaginal epithelial cells (Pubmed).

4. Yu, MA et al (2010). Oxidative stress with an activation of the renin-angiotensin system in human vascular endothelial cells as a novel mechanism of uric acid-induced endothelial dysfunction. J Hypertens 28(6):1234-42. Assay: Uric Acid in Human endothelial cell (Pubmed).

5. Xu, X et al (2011). Major histocompatibility complex class I-related chain A/B (MICA/B) expression in tumor tissue and serum of pancreatic cancer: role of uric acid accumulation in gemcitabine-induced MICA/B expression. BMC Cancer 11(1):194. Assay: Uric Acid in Human PANC-1 pancreatic cancer cell lysate (Pubmed).

6. Kaya, H et al (2008). The protective effect of N-acetylcysteine against cyclosporine A-induced hepatotoxicity in rats. J Appl Toxicol 28(1):15-20. Assay: Uric Acid in Rat Serum (Pubmed).

7. Kamel, AH (2007). Conventional and planar chip sensors for potentiometric assay of uric acid in biological fluids using flow injection analysis. J Pharm Biomed Anal 45(2):341-8. Assay: Uric Acid in Human Urine (Pubmed).

8. Whidden, MA et al (2009). Xanthine oxidase contributes to mechanical ventilation-induced diaphragmatic oxidative stress and contractile dysfunction. J Appl Physiol 106(2):385-94. Assay: Uric Acid in Rat Diaphragm (Pubmed).

9. Moon, HG et al (2010). Conversion of Th17-type into Th2-type inflammation by acetyl salicylic acid via the adenosine and uric acid pathway in the lung. Allergy 65(9):1093-103. Assay: Uric Acid in Mouse lung tissue (Pubmed).

10. Belarbi, M et al (2011). Oleaster Oil Positively Modulates Plasma Lipids in Humans. J Agric Food Chem . Assay: Uric Acid in Human Plasma (Pubmed).

11. Viel, EC et al (2008). Xanthine oxidase and mitochondria contribute to vascular superoxide anion generation in DOCA-salt hypertensive rats. Am J Physiol Heart Circ Physiol 295(1):H281-8. Assay: Uric Acid in Rat Plasma (Pubmed).

12. DiSilvestro RA, DiSilvestro DJ (2009). Pomegranate extract mouth rinsing effects on saliva measures relevant to gingivitis risk. Phytother Res 23(8):1123-7. Assay: Uric Acid in Human Saliva (Pubmed).

13. Karaouzene, N et al (2011). Effects of the association of aging and obesity on lipids, lipoproteins and oxidative stress biomarkers: A comparison of older with young men. Nutr Metab Cardiovasc Dis . Assay: Uric Acid in Human Plasma (Pubmed).

14. Smith, CL et al (2011). Naturally high plasma glucose levels in mourning doves (Zenaida macroura) do not lead to high levels of reactive oxygen species in the vasculature. Zoology (Jena) 114(3):171-6. Assay: Uric Acid in Bird Plasma (mourning dove) (Pubmed).

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