EnzyChrom™ D-Lactate Assay KitEnzyChrom™ D-Lactate Assay Kit
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EnzyChrom™ D-Lactate Assay Kit

For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

• Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay.

• Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed. 

• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems’ EnzyChrom™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

Cat# Size Price Qty Buy
EDLC-100 100 Tests £326.65

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.
Method OD565 nm
Samples Serum, plasma, cell culture media etc
Species All
Detection Limit 0.05 mM 
Storage -20°C
Shelf Life 6 months
References Assay: D-Lactate in Pig Plasma (Pubmed).

2. Gigante A et al (2012) D-Lactic acidosis 25 years after bariatric surgery due to Salmonella enteritidis. Nutrition 28(1):108-11. Assay: D-Lactate in Human Serum (Pubmed).

3. Marcu, R et al (2014). Mitochondrial Matrix Ca2 Accumulation Regulates Cytosolic NAD/ NADH Metabolism, Protein Acetylation, and Sirtuin Expression. Mol Cell Biol. 34(15):2890-902. Assay: Pulmonary Artery Andothelial Cells in Humans (Pubmed).

4. Raycol, Z et al (2014). Myo-inositol trispy rophosphate-mediated hypoxia reversioncontr ols pancreatic cancer in rodents and enhances gemcitabineefficacy. Int J Cancer. 134(11):2572-82. Assay: pancreatic tumor cells in Rats and Mice (Pubmed).

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1. Kurundkar, AR et al (2010). Extracorporeal membrane oxygenation causes loss of intestinal epithelial barrier in the newborn piglet. Pediatr Res 68(2):128-33. Assay: D-Lactate in Pig Plasma (Pubmed).

2. Gigante A et al (2012) D-Lactic acidosis 25 years after bariatric surgery due to Salmonella enteritidis. Nutrition 28(1):108-11. Assay: D-Lactate in Human Serum (Pubmed).

3. Marcu, R et al (2014). Mitochondrial Matrix Ca2 Accumulation Regulates Cytosolic NAD/ NADH Metabolism, Protein Acetylation, and Sirtuin Expression. Mol Cell Biol. 34(15):2890-902. Assay: Pulmonary Artery Andothelial Cells in Humans (Pubmed).

4. Raycol, Z et al (2014). Myo-inositol trispy rophosphate-mediated hypoxia reversioncontr ols pancreatic cancer in rodents and enhances gemcitabineefficacy. Int J Cancer. 134(11):2572-82. Assay: pancreatic tumor cells in Rats and Mice (Pubmed).

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1. Kurundkar, AR et al (2010). Extracorporeal membrane oxygenation causes loss of intestinal epithelial barrier in the newborn piglet. Pediatr Res 68(2):128-33. Assay: D-Lactate in Pig Plasma (Pubmed).

2. Gigante A et al (2012) D-Lactic acidosis 25 years after bariatric surgery due to Salmonella enteritidis. Nutrition 28(1):108-11. Assay: D-Lactate in Human Serum (Pubmed).

3. Marcu, R et al (2014). Mitochondrial Matrix Ca2 Accumulation Regulates Cytosolic NAD/ NADH Metabolism, Protein Acetylation, and Sirtuin Expression. Mol Cell Biol. 34(15):2890-902. Assay: Pulmonary Artery Andothelial Cells in Humans (Pubmed).

4. Raycol, Z et al (2014). Myo-inositol trispy rophosphate-mediated hypoxia reversioncontr ols pancreatic cancer in rodents and enhances gemcitabineefficacy. Int J Cancer. 134(11):2572-82. Assay: pancreatic tumor cells in Rats and Mice (Pubmed).

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