EnzyChrom™ Free Fatty Acid Assay KitEnzyChrom™ Free Fatty Acid Assay Kit
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EnzyChrom™ Free Fatty Acid Assay Kit

For quantitative determination of fatty acid and evaluation of drug effects on its metabolism.

• Sensitive. Use 10 μL samples. Linear detection range: colorimetric assay 7 - 1000 μM, fluorimetric assay 7 - 100 μM fatty acid.

• Convenient. Room temperature "mix-and-read" procedure can be readily automated for high-throughput assay of thousands of samples per day. 

Fatty acids are aliphatic monocarboxylic acids that are ubiquitously found in animal or vegetable fat, oil and wax. Fatty Acids play important roles in cellular synthesis, energy metabolism and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome and Reye Syndrome. BioAssay Systems’ method provides a simple, one-step and high-throughput assay for measuring free fatty acids. In this assay, free fatty acids are enzymatically converted to acyl-CoA and subsequently to H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the free fatty acid concentration in the sample.

Cat# Size Price Qty Buy
EFFA-100 100 Tests £335.15

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of fatty acid and evaluation of drug effects on its metabolism.
Method OD570nm, or FL530/585nm
Samples Serum, plasma, urine, saliva, milk, cell cultures, food, agriculture etc 
Species All
Detection Limit 7 μM
Storage -20°C
Shelf Life 6 months
References Assay: Fatty Acid in Mouse Plasma (Pubmed).

2. Seo, CW et al (2011). Antihyperlipidemic and body fat-lowering effects of silk proteins with different fibroin/sericin compositions in mice fed with high fat diet. J Agric Food Chem 59(8):4192-4197. Assay: Fatty Acid in Mouse Plasma (Pubmed).

3. Primassin, S., S. Tucci, et al. (2011). Hepatic and muscular effects of different dietary fat content in VLCAD deficient mice. Mol Genet Metab 104(4): 546-51. Assay: Fatty Acid in Mouse Serum (Pubmed).

4. Jelinek D et al (2012) The Niemann-Pick C1 Gene Is Downregulated in Livers of C57BL/6J Mice by Dietary Fatty Acids, but Not Dietary Cholesterol, through Feedback Inhibition of the SREBP Pathway. J Nutr 142(11):1935-1942. Assay: Fatty Acid in Mouse Plasma (Pubmed).

5. Jin Kyubob et al (2012) Dysregulation of hepatic fatty acid metabolism in chronic kidney disease. Nephrol Dial Transplant. Assay: Fatty Acid in Rat Liver (Pubmed).

6. Lu Z et al (2012) TLR4 Antagonist Reduces Early-Stage Atherosclerosis in Diabetic Apolipoprotein E-deficient Mice. J Endocrinol . Assay: Fatty Acid in Mouse Serum (Pubmed).

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1. Hwang I et al (2012) Catalase deficiency accelerates diabetic renal injury through peroxisomal dysfunction. Diabetes 61(3):728-738. Assay: Fatty Acid in Mouse Plasma (Pubmed).

2. Seo, CW et al (2011). Antihyperlipidemic and body fat-lowering effects of silk proteins with different fibroin/sericin compositions in mice fed with high fat diet. J Agric Food Chem 59(8):4192-4197. Assay: Fatty Acid in Mouse Plasma (Pubmed).

3. Primassin, S., S. Tucci, et al. (2011). Hepatic and muscular effects of different dietary fat content in VLCAD deficient mice. Mol Genet Metab 104(4): 546-51. Assay: Fatty Acid in Mouse Serum (Pubmed).

4. Jelinek D et al (2012) The Niemann-Pick C1 Gene Is Downregulated in Livers of C57BL/6J Mice by Dietary Fatty Acids, but Not Dietary Cholesterol, through Feedback Inhibition of the SREBP Pathway. J Nutr 142(11):1935-1942. Assay: Fatty Acid in Mouse Plasma (Pubmed).

5. Jin Kyubob et al (2012) Dysregulation of hepatic fatty acid metabolism in chronic kidney disease. Nephrol Dial Transplant. Assay: Fatty Acid in Rat Liver (Pubmed).

6. Lu Z et al (2012) TLR4 Antagonist Reduces Early-Stage Atherosclerosis in Diabetic Apolipoprotein E-deficient Mice. J Endocrinol . Assay: Fatty Acid in Mouse Serum (Pubmed).

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1. Hwang I et al (2012) Catalase deficiency accelerates diabetic renal injury through peroxisomal dysfunction. Diabetes 61(3):728-738. Assay: Fatty Acid in Mouse Plasma (Pubmed).

2. Seo, CW et al (2011). Antihyperlipidemic and body fat-lowering effects of silk proteins with different fibroin/sericin compositions in mice fed with high fat diet. J Agric Food Chem 59(8):4192-4197. Assay: Fatty Acid in Mouse Plasma (Pubmed).

3. Primassin, S., S. Tucci, et al. (2011). Hepatic and muscular effects of different dietary fat content in VLCAD deficient mice. Mol Genet Metab 104(4): 546-51. Assay: Fatty Acid in Mouse Serum (Pubmed).

4. Jelinek D et al (2012) The Niemann-Pick C1 Gene Is Downregulated in Livers of C57BL/6J Mice by Dietary Fatty Acids, but Not Dietary Cholesterol, through Feedback Inhibition of the SREBP Pathway. J Nutr 142(11):1935-1942. Assay: Fatty Acid in Mouse Plasma (Pubmed).

5. Jin Kyubob et al (2012) Dysregulation of hepatic fatty acid metabolism in chronic kidney disease. Nephrol Dial Transplant. Assay: Fatty Acid in Rat Liver (Pubmed).

6. Lu Z et al (2012) TLR4 Antagonist Reduces Early-Stage Atherosclerosis in Diabetic Apolipoprotein E-deficient Mice. J Endocrinol . Assay: Fatty Acid in Mouse Serum (Pubmed).

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