Tubulin Polymerization Assay (fluoresc-96assays porcine brain tubulin)Tubulin Polymerization Assay (fluoresc-96assays porcine brain tubulin)
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Tubulin Polymerization Assay (fluoresc-96assays porcine brain tubulin)

Product Uses Include

  • Cost effective high throughput screen for anti-cancer compounds.
  • Basic research to measure compound IC50s and specificity for tubulin.
  • Screening proteins for effects on tubulin polymerization activity.
  • Teaching aid for undergraduate/graduate class in pharmacology.

Introduction
This assay is an economical one step procedure for determining the effects of drugs or proteins on tubulin polymerization. It is an adaptation of an assay originally described by Bonne, D. et al. (1).  Polymerization is followed by fluorescence enhancement due to the incorporation of a fluorescent reporter into microtubules as polymerization occurs. The standard assay uses neuronal tubulin (Cat. # T240), which generates a polymerization curve representing the three phases of microtubule formation; namely nucleation, growth and steady state equilibrium.  Other tubulins, such as HeLa cell tubulin (Cat. # H001) can also be used in this assay.  The low volume per assay of 50 µl and the low tubulin concentrations, 2 mg/ml final concentration (100 µg per assay), makes this an ideal choice for studying the more expensive cancer cell tubulin reagents and for high throughput applications.

The classic tubulin polymerization assay uses absorbance readings at 340 nm to follow microtubule formation.  It is based upon the fact that light is scattered by microtubules to an extent that is proportional to the concentration of microtubule polymer. This assay is offered by Cytoskeleton, Inc. (Cat. # BK006P).  

Kit contents
The kit contains sufficient material for 96 assays in 50 µl format. The following components are included:

  1. >99% pure tubulin (Cat. # T240)
  2. General tubulin buffer with fluorescent reporter
  3. Microtubule glycerol buffer (Cat. # BST05)
  4. GTP solution (Cat. # BST06)
  5. Paclitaxel positive control (Cat. # TXD01)
  6. Half area 96-well plate. Black, flat bottom
  7. Manual with detailed protocols and extensive troubleshooting guide

Equipment needed

  1. Tempererature controlled 96-well plate fluorimeter equipped with filters for exitation at 340-360 nm and emission at 420-450 nm
Cat# Size Price Qty Buy
BK011P 96 assays £777.66

Additional Information

Property Value or Rating
Product Size 96 assays
Manufacturer Cytoskeleton, Inc
Storage 4°C

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