QuantiChrom™ Boron Assay Kit

Application

• Quantitative determination of boron in agricultural and environmental samples.

Key Features

• Fast and sensitive. Linear detection range: 0.05 to 10 µg/mL (0.05 - 10 ppm) boron with 100 µL sample (96-well).
• Convenient. The procedure involves adding a single working reagent.
• High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day.

Method

•  OD420nm

Samples

•  Water, plant tisse, soil samples, and antibody conjugation solutions.

Species

•  All

Size

•  100 tests

Detection Limit

•  0.05 μg/mL or 0.05 ppm

Shelf Life

•  12 months

More Details

•  BORON is an essential micronutrient in plants and is involved in maintaining robust cell walls, cell membranes, and reproductive tissues. Although boron is common in the soil in its natural state as a borate mineral, the amount of boron available to plants is actually quite small. As a result, boron deficiency is the second most common micronutrient deficiency among crop plants. In order to keep plant boron levels in a healthy range, supplementation to the soil via fertilizers and additives is often required. If not regulated, a lack of or excess of boron may significantly lower crop yield. In the biotech industry, sodium borohydride is commonly used to conjugate antibodies and typically needs to be removed from the final product, especially for therapeutic antibodies.
  
  BioAssay Systems' boron detection kit provides a convenient and reliable means to measure boron. In the assay borate complexes with azomethine-H to create a colored compound that can be measured at 420 nm. This assay can be used with a variety of samples and is simple, sensitive, and adaptable to high-throughput screening.