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Neutral Protease AF GMP Grade

For Cell Isolation from Pancreas, Adipose Tissue and Tumors

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Neutral Protease AF GMP Grade is derived from C.histolyticum using a production process completely free of any animal-based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This purified metalloprotease is suitable for the dissociation of tissue in clinical applications. For especially gentle cell isolation it is recommended to use Neutral Protease AF GMP Grade in combination with highly purified Collagenase AF-1 GMP Grade. Here, it has been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation and stromal vascular fraction (SVF) from adipose tissue and tumor cells.

Cat#	Size	Price	Qty	Buy
N0003553	≥ 100 U (DMC)	£505.82

Additional Information

Property	Value or Rating
Product Size	≥ 100 U (DMC)
Manufacturer	Nordmark Biochemicals
Storage	+2 °C to +8 °C
References	References and Definitions Unit definition: Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1). Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)]. References: 1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93

References and Definitions

Unit definition:
Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).
Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)].

References:
1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93

Neutral Protease AF GMP Grade

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Neutral Protease AF GMP Grade

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