Metal Chelate Midi Kit - 8 MC Plugs includes buffers

The Proteus IMAC kit is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions. Proteus spin columns replace lengthy and expensive chromatographic methods such as FPLC. Metal chelate affinity chromatography is a rapid one step purification, which removes most contaminants and can achieve purities close to homogeneity. Contents: Quantity: 8 x 1.6 ml Ni-IMAC columns Max. sample volume per load: 20 ml, swing bucket rotor Collection tube: 50 ml centrifuge tubes Min. number of purifications: 16 purifications (2 uses per column) Typical capacity/preparation: 10 - 15 mg 6 x His-tagged protein Vivaspin 20 ultrafiltration concentrators: 8 Buffers: 5 x PBS Buffer A, 1 x Imidazole Buffer B