Actin Thin Filaments (Rabbit skeletal)

Product Uses - Measurement of calcium activated myosin ATPase activity when bound to thin filaments. - Identification/characterization of proteins or small molecules that affect the SMTFC regulation and myosin ATPase activity - Identification/characterization of proteins or small molecules that affect myosin / F-actin interaction Materials The skeletal muscle thin filament complex (SMTFC) has been assembled from purified F-actin (Cat. # AKL99) and Tropomyosin / Troponin protein (TT) complex (Cat. # TT06). Thus, SMTFC is composed of six proteins: Actin : Tropomyosin α: Tropomyosin β : Troponin C : Troponin I : Troponin T in a stoichiometric ratio of 7:1:1:1:1:1; see Figure 1. After assembly, the thin filaments were centrifuged at 100,000 x g and the pellets resuspended in reaction buffer which purifies the calcium sensitive complex. The complex has been determined to be biologically active in a calcium activat-ed myosin ATPase assay (see biological activity assay). The complex is supplied as a white lyophilized powder.