SiR700-DNA

SiR700-DNA is based on the far-red silicon rhodamine (SiR) fluorophore analogue SiR700 and the DNA minor groove binder bisbenzimide (Hoechst). SiR700-DNA allows the labelling of DNA in live cells with high specificity and low background without the need for genetic manipulation or over-expression. The key features of SiR700-DNA are i) far-red absorption and emission wavelengths, ii) cell permeability, iii) fluorogenic character, and iv) compatibility with super-resolution microscopy (e.g., STED & SIM). In addition, SiR700-DNA can be used for wide-field and confocal fluorescent imaging in living cells. The emission in the far-red wavelength minimizes phototoxicity and sample autofluorescence. SiR700-DNA is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filter sets. This probe has multiple advantages (far-red, fluorogenic, cell permeable, low background, and high specificity for DNA) over existing probes such Syto61, DRAQ5, and Vybrant DyeCycle Ruby dyes. Probe quantity allows 35 – 140 staining experiments.* 

Optical properties

λ_abs    689 nm
λ_ex      716 nm

ε_max   1.0·10⁵ mol^-1·cm^-1

MW     974.2 g/mol

MF      C₆₀H₆₅N₉O₄Si 

*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 µM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.