Product Uses Include
- Rho signaling pathway studies
- Rho activation assays with primary cells
- Studies of Rho activators and inactivators
- Rho activation assays with limited material
- High throughput screens for Rho activation
Introduction
This G-LISA™ Rho activation assay measures the levels of GTP-loaded RhoA in cells. The level of activation is measured with luminometry. The G-LISA Rho activation assays are ELISA based Rho activation assays with wich you can measure Rho activity in cells in less than 3 h. For a kit to measure RhoA activation with colorimetric detection, see Cat. # BK124
Kit contents
The kit contains sufficient reagents to perform 96 Rho activation assays. Since the Rho-GTP affinity wells are supplied as strips and the strips can be broken into smaller pieces, each kit can be used for anywhere from one to multiple assays. The following components are included in the kit:
- 96 Rho-GTP affinity wells
- Lysis buffer
- Binding buffer
- Antigen presenting buffer
- Wash buffer
- Antibody dilution buffer
- Anti-RhoA antibody
- HRP-labeled secondary antibody
- Positive control RhoA protein
- Protease inhibitor cocktail (Cat. # PIC02)
- Luminescence detection reagents
- Precision Red™ Advanced protein assay reagent (Cat. # ADV02)
- Manual with detailed protocols and extensive troubleshooting guide
Equipment needed
- Luminometer capable of reading a 96-well plate
- Multichannel or multidispensing pipettor
- Orbital microplate shaker capable of at least 200 rpm shaking (400 rpm is optimal)
