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QuantiChrom™ Peroxide Assay Kit

For quantitative determination of peroxide and evaluation of drug effects on peroxide metabolism.

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• Sensitive and accurate. Enhanced color intensity using sorbitol. Detection range 0.2 μM (7 ng/mL) to 30 μM (1,020 ng/mL) H2O2 in 96-well plate assay.

• Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.

Peroxide (e.g. hydrogen peroxide H2O2) is one of the key reactive oxygen species formed under oxidative stress conditions. High levels of peroxide formation have been linked to pathological conditions such as ageing, asthma, diabetes, atherosclerosis, cataract, inflammatory arthritis and neurodegenerative diseases. Simple, direct and automation-ready procedures for quantitative determination of peroxide find wide applications in research and drug discovery. BioAssay Systems’ peroxide assay kit is designed to measure peroxide concentration in biological samples without any pretreatment. The improved method utilizes the chromogenic Fe3+-xylenol orange reaction, in which a purple complex is formed when Fe2+ provided in the reagent is oxidized to Fe3+ by peroxides present in the sample. The intensity of the color, measured at 540-610nm, is an accurate measure of the peroxide level in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

Cat#	Size	Price	Qty	Buy
DIOX-250	250 Tests	£245.65

Additional Information

Property	Value or Rating
Product Size	250 Tests
Manufacturer	BioAssay Systems
Applications	For quantitative determination of peroxide and evaluation of drug effects on peroxide metabolism.
Method	OD585nm
Samples	Serum, citrate-plasma, urine, cell lysate, culture media etc
Species	All
Detection Limit	0.2 μM
Storage	4°C
Shelf Life	6 months
References	Assay: Peroxide in Mouse Macrophages, culture supernatant (Pubmed). 2. Zhang, R et al (2008). In vitro and in vivo induction of apoptosis by capsaicin in pancreatic cancer cells is mediated through ROS generation and mitochondrial death pathway. Apoptosis 13(12):1465-78. Assay: Peroxide in Human pancreatic cancer cell (Pubmed). 3. Deshmane, SL et al (2009). Activation of the oxidative stress pathway by HIV-1 Vpr leads to induction of hypoxia-inducible factor 1alpha expression. J Biol Chem 284(17):11364-73. Assay: Peroxide in Human Microglia cell line (Pubmed). 4. Cunningham, RL et al (2009). Androgens induce dopaminergic neurotoxicity via caspase-3-dependent activation of protein kinase Cdelta. Endocrinology 150(12):5539-48. Assay: Peroxide in Rat Dopaminergic cell line N27 (Pubmed). 5. Toschi, A et al (2010). Phospholipase D-mTOR requirement for the Warburg effect in human cancer cells. Cancer Lett 299(1):72-9. Assay: Peroxide in Human cells (Pubmed). 6. Fedeles, BI et al (2011). Chemical genetics analysis of an aniline mustard anticancer agent reveals complex I of the electron transport chain as a target. J Biol Chem . Assay: Peroxide in Human cell lines, HeLa cells (Pubmed). 7. Hashimoto, T et al (2007). Lactate sensitive transcription factor network in L6 cells: activation of MCT1 and mitochondrial biogenesis. FASEB J 21(10):2602-12. Assay: Peroxide in Rat L6 cell lysate (Pubmed). 8. Yu, CL et al (2008). A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid. J Bacteriol 190(2):772-6. Assay: Peroxide in Bacteria Purified enzyme (Pseudomonas sp.) (Pubmed). 9. Minamishima, S et al (2009). Hydrogen sulfide improves survival after cardiac arrest and cardiopulmonary resuscitation via a nitric oxide synthase 3-dependent mechanism in mice. Circulation 120(10):888-96. Assay: Peroxide in Mouse Serum (Pubmed). 10. Shin, D et al (2009). Dicer ablation in oligodendrocytes provokes neuronal impairment in mice. Ann Neurol 66(6):843-57. Assay: Peroxide in Mouse Brain tissue (Pubmed). 11. Fan, X et al (2010). H2O2-induced mitochondrial fragmentation in C2C12 myocytes. Free Radic Biol Med 49(11):1646-54. Assay: Peroxide in Mouse Cell culture supernatant (Pubmed). 12. Nguyen GN et al (2009) Drought-Induced Oxidative Conditions in Rice Anthers Leading to a Programmed Cell Death and Pollen Abortion. Journal of Agronomy and Crop Science 195(3):157-164. Assay: Peroxide in Plant Rice anther (Pubmed). 13. Lens, M et al (2008). Antioxidative capacity of C(60) (buckminsterfullerene) and newly synthesized fulleropyrrolidine derivatives encapsulated in liposomes. Biotechnol Appl Biochem 51(Pt 3):135-40. Assay: Peroxide in N/A Fullerene liposomes (Pubmed). 14. Zhang, H et al (2009). Resveratrol improves endothelial function: role of TNF{alpha} and vascular oxidative stress. Arterioscler Thromb Vasc Biol 29(8):1164-71. Assay: Peroxide in Mouse Serum (Pubmed). 15. Schnabl, KL et al (2009). Gangliosides protect bowel in an infant model of necrotizing enterocolitis by suppressing proinflammatory signals. J Pediatr Gastroenterol Nutr 49(4):382-92. Assay: Peroxide in Human Intestinal tissue (Pubmed). 16. Jawed, H et al (2010). N-(2-hydroxy phenyl) acetamide inhibits inflammation-related cytokines and ROS in adjuvant-induced arthritic (AIA) rats. Int Immunopharmacol 10(8):900-5. Assay: Peroxide in Rat Plasma/Serum (Pubmed). 17. Peng, A et al (2011). The green tea polyphenol (-)-epigallocatechin-3-gallate ameliorates experimental immune-mediated glomerulonephritis. Kidney Int . Assay: Peroxide in Mouse Kidney tissue, Urine, Serum (Pubmed). " title=" 1. Sato, T et al (2008). Suppressive oligodeoxynucleotides inhibit silica-induced pulmonary inflammation. J Immunol 180(11):7648-54. Assay: Peroxide in Mouse Macrophages, culture supernatant (Pubmed). 2. Zhang, R et al (2008). In vitro and in vivo induction of apoptosis by capsaicin in pancreatic cancer cells is mediated through ROS generation and mitochondrial death pathway. Apoptosis 13(12):1465-78. Assay: Peroxide in Human pancreatic cancer cell (Pubmed). 3. Deshmane, SL et al (2009). Activation of the oxidative stress pathway by HIV-1 Vpr leads to induction of hypoxia-inducible factor 1alpha expression. J Biol Chem 284(17):11364-73. Assay: Peroxide in Human Microglia cell line (Pubmed). 4. Cunningham, RL et al (2009). Androgens induce dopaminergic neurotoxicity via caspase-3-dependent activation of protein kinase Cdelta. Endocrinology 150(12):5539-48. Assay: Peroxide in Rat Dopaminergic cell line N27 (Pubmed). 5. Toschi, A et al (2010). Phospholipase D-mTOR requirement for the Warburg effect in human cancer cells. Cancer Lett 299(1):72-9. Assay: Peroxide in Human cells (Pubmed). 6. Fedeles, BI et al (2011). Chemical genetics analysis of an aniline mustard anticancer agent reveals complex I of the electron transport chain as a target. J Biol Chem . Assay: Peroxide in Human cell lines, HeLa cells (Pubmed). 7. Hashimoto, T et al (2007). Lactate sensitive transcription factor network in L6 cells: activation of MCT1 and mitochondrial biogenesis. FASEB J 21(10):2602-12. Assay: Peroxide in Rat L6 cell lysate (Pubmed). 8. Yu, CL et al (2008). A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid. J Bacteriol 190(2):772-6. Assay: Peroxide in Bacteria Purified enzyme (Pseudomonas sp.) (Pubmed). 9. Minamishima, S et al (2009). Hydrogen sulfide improves survival after cardiac arrest and cardiopulmonary resuscitation via a nitric oxide synthase 3-dependent mechanism in mice. Circulation 120(10):888-96. Assay: Peroxide in Mouse Serum (Pubmed). 10. Shin, D et al (2009). Dicer ablation in oligodendrocytes provokes neuronal impairment in mice. Ann Neurol 66(6):843-57. Assay: Peroxide in Mouse Brain tissue (Pubmed). 11. Fan, X et al (2010). H2O2-induced mitochondrial fragmentation in C2C12 myocytes. Free Radic Biol Med 49(11):1646-54. Assay: Peroxide in Mouse Cell culture supernatant (Pubmed). 12. Nguyen GN et al (2009) Drought-Induced Oxidative Conditions in Rice Anthers Leading to a Programmed Cell Death and Pollen Abortion. Journal of Agronomy and Crop Science 195(3):157-164. Assay: Peroxide in Plant Rice anther (Pubmed). 13. Lens, M et al (2008). Antioxidative capacity of C(60) (buckminsterfullerene) and newly synthesized fulleropyrrolidine derivatives encapsulated in liposomes. Biotechnol Appl Biochem 51(Pt 3):135-40. Assay: Peroxide in N/A Fullerene liposomes (Pubmed). 14. Zhang, H et al (2009). Resveratrol improves endothelial function: role of TNF{alpha} and vascular oxidative stress. Arterioscler Thromb Vasc Biol 29(8):1164-71. Assay: Peroxide in Mouse Serum (Pubmed). 15. Schnabl, KL et al (2009). Gangliosides protect bowel in an infant model of necrotizing enterocolitis by suppressing proinflammatory signals. J Pediatr Gastroenterol Nutr 49(4):382-92. Assay: Peroxide in Human Intestinal tissue (Pubmed). 16. Jawed, H et al (2010). N-(2-hydroxy phenyl) acetamide inhibits inflammation-related cytokines and ROS in adjuvant-induced arthritic (AIA) rats. Int Immunopharmacol 10(8):900-5. Assay: Peroxide in Rat Plasma/Serum (Pubmed). 17. Peng, A et al (2011). The green tea polyphenol (-)-epigallocatechin-3-gallate ameliorates experimental immune-mediated glomerulonephritis. Kidney Int . Assay: Peroxide in Mouse Kidney tissue, Urine, Serum (Pubmed). " target="_blank"> 1. Sato, T et al (2008). Suppressive oligodeoxynucleotides inhibit silica-induced pulmonary inflammation. J Immunol 180(11):7648-54. Assay: Peroxide in Mouse Macrophages, culture supernatant (Pubmed). 2. Zhang, R et al (2008). In vitro and in vivo induction of apoptosis by capsaicin in pancreatic cancer cells is mediated through ROS generation and mitochondrial death pathway. Apoptosis 13(12):1465-78. Assay: Peroxide in Human pancreatic cancer cell (Pubmed). 3. Deshmane, SL et al (2009). Activation of the oxidative stress pathway by HIV-1 Vpr leads to induction of hypoxia-inducible factor 1alpha expression. J Biol Chem 284(17):11364-73. Assay: Peroxide in Human Microglia cell line (Pubmed). 4. Cunningham, RL et al (2009). Androgens induce dopaminergic neurotoxicity via caspase-3-dependent activation of protein kinase Cdelta. Endocrinology 150(12):5539-48. Assay: Peroxide in Rat Dopaminergic cell line N27 (Pubmed). 5. Toschi, A et al (2010). Phospholipase D-mTOR requirement for the Warburg effect in human cancer cells. Cancer Lett 299(1):72-9. Assay: Peroxide in Human cells (Pubmed). 6. Fedeles, BI et al (2011). Chemical genetics analysis of an aniline mustard anticancer agent reveals complex I of the electron transport chain as a target. J Biol Chem . Assay: Peroxide in Human cell lines, HeLa cells (Pubmed). 7. Hashimoto, T et al (2007). Lactate sensitive transcription factor network in L6 cells: activation of MCT1 and mitochondrial biogenesis. FASEB J 21(10):2602-12. Assay: Peroxide in Rat L6 cell lysate (Pubmed). 8. Yu, CL et al (2008). A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid. J Bacteriol 190(2):772-6. Assay: Peroxide in Bacteria Purified enzyme (Pseudomonas sp.) (Pubmed). 9. Minamishima, S et al (2009). Hydrogen sulfide improves survival after cardiac arrest and cardiopulmonary resuscitation via a nitric oxide synthase 3-dependent mechanism in mice. Circulation 120(10):888-96. Assay: Peroxide in Mouse Serum (Pubmed). 10. Shin, D et al (2009). Dicer ablation in oligodendrocytes provokes neuronal impairment in mice. Ann Neurol 66(6):843-57. Assay: Peroxide in Mouse Brain tissue (Pubmed). 11. Fan, X et al (2010). H2O2-induced mitochondrial fragmentation in C2C12 myocytes. Free Radic Biol Med 49(11):1646-54. Assay: Peroxide in Mouse Cell culture supernatant (Pubmed). 12. Nguyen GN et al (2009) Drought-Induced Oxidative Conditions in Rice Anthers Leading to a Programmed Cell Death and Pollen Abortion. Journal of Agronomy and Crop Science 195(3):157-164. Assay: Peroxide in Plant Rice anther (Pubmed). 13. Lens, M et al (2008). Antioxidative capacity of C(60) (buckminsterfullerene) and newly synthesized fulleropyrrolidine derivatives encapsulated in liposomes. Biotechnol Appl Biochem 51(Pt 3):135-40. Assay: Peroxide in N/A Fullerene liposomes (Pubmed). 14. Zhang, H et al (2009). Resveratrol improves endothelial function: role of TNF{alpha} and vascular oxidative stress. Arterioscler Thromb Vasc Biol 29(8):1164-71. Assay: Peroxide in Mouse Serum (Pubmed). 15. Schnabl, KL et al (2009). Gangliosides protect bowel in an infant model of necrotizing enterocolitis by suppressing proinflammatory signals. J Pediatr Gastroenterol Nutr 49(4):382-92. Assay: Peroxide in Human Intestinal tissue (Pubmed). 16. Jawed, H et al (2010). N-(2-hydroxy phenyl) acetamide inhibits inflammation-related cytokines and ROS in adjuvant-induced arthritic (AIA) rats. Int Immunopharmacol 10(8):900-5. Assay: Peroxide in Rat Plasma/Serum (Pubmed). 17. Peng, A et al (2011). The green tea polyphenol (-)-epigallocatechin-3-gallate ameliorates experimental immune-mediated glomerulonephritis. Kidney Int . Assay: Peroxide in Mouse Kidney tissue, Urine, Serum (Pubmed).

Related Documents

1. Sato, T et al (2008). Suppressive oligodeoxynucleotides inhibit silica-induced pulmonary inflammation. J Immunol 180(11):7648-54. Assay: Peroxide in Mouse Macrophages, culture supernatant (Pubmed).

2. Zhang, R et al (2008). In vitro and in vivo induction of apoptosis by capsaicin in pancreatic cancer cells is mediated through ROS generation and mitochondrial death pathway. Apoptosis 13(12):1465-78. Assay: Peroxide in Human pancreatic cancer cell (Pubmed).

3. Deshmane, SL et al (2009). Activation of the oxidative stress pathway by HIV-1 Vpr leads to induction of hypoxia-inducible factor 1alpha expression. J Biol Chem 284(17):11364-73. Assay: Peroxide in Human Microglia cell line (Pubmed).

4. Cunningham, RL et al (2009). Androgens induce dopaminergic neurotoxicity via caspase-3-dependent activation of protein kinase Cdelta. Endocrinology 150(12):5539-48. Assay: Peroxide in Rat Dopaminergic cell line N27 (Pubmed).

5. Toschi, A et al (2010). Phospholipase D-mTOR requirement for the Warburg effect in human cancer cells. Cancer Lett 299(1):72-9. Assay: Peroxide in Human cells (Pubmed).

6. Fedeles, BI et al (2011). Chemical genetics analysis of an aniline mustard anticancer agent reveals complex I of the electron transport chain as a target. J Biol Chem . Assay: Peroxide in Human cell lines, HeLa cells (Pubmed).

7. Hashimoto, T et al (2007). Lactate sensitive transcription factor network in L6 cells: activation of MCT1 and mitochondrial biogenesis. FASEB J 21(10):2602-12. Assay: Peroxide in Rat L6 cell lysate (Pubmed).

8. Yu, CL et al (2008). A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid. J Bacteriol 190(2):772-6. Assay: Peroxide in Bacteria Purified enzyme (Pseudomonas sp.) (Pubmed).

9. Minamishima, S et al (2009). Hydrogen sulfide improves survival after cardiac arrest and cardiopulmonary resuscitation via a nitric oxide synthase 3-dependent mechanism in mice. Circulation 120(10):888-96. Assay: Peroxide in Mouse Serum (Pubmed).

10. Shin, D et al (2009). Dicer ablation in oligodendrocytes provokes neuronal impairment in mice. Ann Neurol 66(6):843-57. Assay: Peroxide in Mouse Brain tissue (Pubmed).

11. Fan, X et al (2010). H2O2-induced mitochondrial fragmentation in C2C12 myocytes. Free Radic Biol Med 49(11):1646-54. Assay: Peroxide in Mouse Cell culture supernatant (Pubmed).

12. Nguyen GN et al (2009) Drought-Induced Oxidative Conditions in Rice Anthers Leading to a Programmed Cell Death and Pollen Abortion. Journal of Agronomy and Crop Science 195(3):157-164. Assay: Peroxide in Plant Rice anther (Pubmed).

13. Lens, M et al (2008). Antioxidative capacity of C(60) (buckminsterfullerene) and newly synthesized fulleropyrrolidine derivatives encapsulated in liposomes. Biotechnol Appl Biochem 51(Pt 3):135-40. Assay: Peroxide in N/A Fullerene liposomes (Pubmed).

14. Zhang, H et al (2009). Resveratrol improves endothelial function: role of TNF{alpha} and vascular oxidative stress. Arterioscler Thromb Vasc Biol 29(8):1164-71. Assay: Peroxide in Mouse Serum (Pubmed).

15. Schnabl, KL et al (2009). Gangliosides protect bowel in an infant model of necrotizing enterocolitis by suppressing proinflammatory signals. J Pediatr Gastroenterol Nutr 49(4):382-92. Assay: Peroxide in Human Intestinal tissue (Pubmed).

16. Jawed, H et al (2010). N-(2-hydroxy phenyl) acetamide inhibits inflammation-related cytokines and ROS in adjuvant-induced arthritic (AIA) rats. Int Immunopharmacol 10(8):900-5. Assay: Peroxide in Rat Plasma/Serum (Pubmed).

17. Peng, A et al (2011). The green tea polyphenol (-)-epigallocatechin-3-gallate ameliorates experimental immune-mediated glomerulonephritis. Kidney Int . Assay: Peroxide in Mouse Kidney tissue, Urine, Serum (Pubmed).

QuantiChrom™ Peroxide Assay Kit

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