QuantiChrom™ Peroxidase Assay Kit (replacement for DPOD-100)QuantiChrom™ Peroxidase Assay Kit (replacement for DPOD-100)
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QuantiChrom™ Peroxidase Assay Kit (replacement for DPOD-100)

For quantitative determination of peroxidase activity. 

• Fast and sensitive. Use as little as 10 μL samples. Linear detection range: colorimetric assays 2 to 50 U/L, fluorimetric assays 0.1 to 5 U/L peroxidase.

• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

PEROXIDASES (EC number 1.11.1.x) catalyze the following oxidation-reduction reactions:  ROOR’ + electron donor (2 e-) + 2H+ → ROH + R’OH.  For many peroxidases the optimal substrate is hydrogen peroxide (H2O2), but others are more active with organic hydroperoxides such as lipid peroxides. In the cell, peroxidases destroy toxic hydroxide radicals that are formed as byproducts during aerobic respiration. The peroxidases represent a large family of enzymes that are found in animals (e.g. myeloperoxidase-like enzymes), plant, fungi and bacteria (cytochrome-c peroxidase like enzymes such a horseradish peroxidase). Simple, direct and automation-ready procedures for determining peroxidase activity find wide applications. BioAssay Systems’ peroxidase assay uses H2O2 and an electron donor dye that forms a pink color during the peroxidase reaction. The optical density (570nm) or fluorescence intensity (λex/em = 530/590nm) is a direct measure of the enzyme activity.

Cat# Size Price Qty Buy
D2PD-100 100 Tests £237.15

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of peroxidase activity. 
Method OD570nm, or FL530/585nm
Samples Biological
Species All
Detection Limit OD, FL: 4, 0.8 U/L
Storage 4, -20°C
Shelf Life 12 months
References Assay: Peroxidase in rat serum (Pubmed).

2. Yu CL et al (2008) A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid. J Bacteriol 190(2):772-6 Assay: Peroxidase in bacterial cell (Pubmed).

3. Nguyen GN et al (2009) Drought-Induced Oxidative Conditions in Rice Anthers Leading to a Programmed Cell Death and Pollen Abortion. Journal of Agronomy and Crop Science 195(3):157-164 Assay: Peroxidase in plant rice anther (Pubmed).

4. Nguyen GN et al (2009) Drought-Induced Oxidative Conditions in Rice Anthers Leading to a Programmed Cell Death and Pollen Abortion. Journal of Agronomy and Crop Science 195(3):157-164. Assay: Peroxidase in plant rice anther (Pubmed).

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1. Jawed H et al (2010) N-(2-hydroxy phenyl) acetamide inhibits inflammation-related cytokines and ROS in adjuvant-induced arthritic (AIA) rats. Int Immunopharmacol 10(8):900-5 Assay: Peroxidase in rat serum (Pubmed).

2. Yu CL et al (2008) A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid. J Bacteriol 190(2):772-6 Assay: Peroxidase in bacterial cell (Pubmed).

3. Nguyen GN et al (2009) Drought-Induced Oxidative Conditions in Rice Anthers Leading to a Programmed Cell Death and Pollen Abortion. Journal of Agronomy and Crop Science 195(3):157-164 Assay: Peroxidase in plant rice anther (Pubmed).

4. Nguyen GN et al (2009) Drought-Induced Oxidative Conditions in Rice Anthers Leading to a Programmed Cell Death and Pollen Abortion. Journal of Agronomy and Crop Science 195(3):157-164. Assay: Peroxidase in plant rice anther (Pubmed).

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1. Jawed H et al (2010) N-(2-hydroxy phenyl) acetamide inhibits inflammation-related cytokines and ROS in adjuvant-induced arthritic (AIA) rats. Int Immunopharmacol 10(8):900-5 Assay: Peroxidase in rat serum (Pubmed).

2. Yu CL et al (2008) A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid. J Bacteriol 190(2):772-6 Assay: Peroxidase in bacterial cell (Pubmed).

3. Nguyen GN et al (2009) Drought-Induced Oxidative Conditions in Rice Anthers Leading to a Programmed Cell Death and Pollen Abortion. Journal of Agronomy and Crop Science 195(3):157-164 Assay: Peroxidase in plant rice anther (Pubmed).

4. Nguyen GN et al (2009) Drought-Induced Oxidative Conditions in Rice Anthers Leading to a Programmed Cell Death and Pollen Abortion. Journal of Agronomy and Crop Science 195(3):157-164. Assay: Peroxidase in plant rice anther (Pubmed).

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