QuantiChrom™ Glyoxalase I Assay KitQuantiChrom™ Glyoxalase I Assay Kit
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QuantiChrom™ Glyoxalase I Assay Kit

For quantitative determination of glyoxalase activity. Note: use a UV plate (e.g. 96-well UV plate, cat# P96UV).

• Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction.

• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

GLYOXALASE I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S-lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methylglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans and human cancer. Simple, direct and automation-ready procedures for measuring GLO-1 activity in biological samples are highly desirable in research and drug discovery. BioAssay Systems’ Glyoxalase I assay kit provides a sensitive and convenient method for GLO-1 activity determination. The method involves monitoring the increase in the product of the GLO-1 reaction, S-lactoylglutathione, by measuring the change in absorbance at 240 nm.

Cat# Size Price Qty Buy
DGLO-100 100 Tests £279.65

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of glyoxalase activity. Note: use a UV plate (e.g. 96-well UV plate, cat# P96UV).
Method OD240nm
Samples Enzyme preparations or biological samples
Species All
Detection Limit 4 U/L
Storage -20°C, RT
Shelf Life 12 months

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