QuantiChrom™ Alkaline Phosphatase Assay KitQuantiChrom™ Alkaline Phosphatase Assay Kit
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QuantiChrom™ Alkaline Phosphatase Assay Kit

For quantitative determination of alkaline phosphatase (ALP) activity using stable p-nitrophenol phosphate substrate.

• High sensitivity and wide linear range. Use 5 μL serum or plasma sample. The detection limit is 2 U/L, linear up to 800 U/L.

• Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 5 minutes.

• Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis. Simple, direct and automation-ready procedures for measuring ALP activity in serum are becoming popular in Research and Drug Discovery. BioAssay Systems’ QuantiChrom™ Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

Cat# Size Price Qty Buy
DALP-250 250 Tests £245.65

Additional Information

Property Value or Rating
Product Size 250 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of alkaline phosphatase (ALP) activity using stable p-nitrophenol phosphate substrate.
Method OD405nm
Samples Serum, plasma etc
Species All
Detection Limit 2 U/L
Storage 4, -20°C
Shelf Life 12 months
References Assay: ALP in Mouse Serum (Pubmed).

2. Sekiya, S, Suzuki, A (2011). Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors. Nature 475(7356):390-3. Assay: ALP in Mouse Plasma (Pubmed).

3. Kim, HJ et al (2006). Glucocorticoids suppress bone formation via the osteoclast. J Clin Invest. 116(8):2152-60. Assay: ALP in Mouse Serum (Pubmed).

4. Panizo, S et al (2009). RANKL increases vascular smooth muscle cell calcification through a RANK-BMP4-dependent pathway. Circ Res. 104(9):1041-8. Assay: ALP in Rat Vascular smooth muscle cells, lysate (Pubmed).

5. Wang, Y et al (2011). Small interfering RNA knocks down the molecular target of alendronate, farnesyl pyrophosphate synthase, in osteoclast and osteoblast cultures. Mol Pharm. 8(4):1016-24. Assay: ALP in Mouse primary osteoblast cells (Pubmed).

6. Bhattacharya, A et al (2006). Effect of fish oil on bone mineral density in aging C57BL/6 female mice. J. Nutr. Biochem 18(6):372-379. Assay: ALP in Mouse serum (Pubmed).

7. Lian, Q et al (2006). Establishing clonal cell lines with endothelial-like potential from CD9(hi), SSEA-1(-) cells in embryonic stem cell-derived embryoid bodies. PLoS One 1:e6. Assay: ALP in Mouse Stem cell line (Pubmed).

8. Henderson, JA et al (2008). Concurrent differentiation of marrow stromal cells to osteogenic and vasculogenic lineages. Macromol Biosci. 8(6):499-507. Assay: ALP in Rat bone marrow stromal cells (Pubmed).

9. Keskar, V et al (2009). In vitro evaluation of macroporous hydrogels to facilitate stem cell infiltration, growth, and mineralization. Tissue Eng Part A. 15(7):1695-707. Assay: ALP in Human Mesenchymal stem cells grown on hydrogels (Pubmed).

10. Oldinski, RA et al (2010). Synthesis and characterization of a Hyaluronan-polyethylene copolymer for biomedical applications. J Biomed Mater Res B Appl Biomater. 94(2):441-6. Assay: ALP in Human Cells (Pubmed).

11. Gaharwar, AK et al (2011). Assessment of using laponite cross-linked poly(ethylene oxide) for controlled cell adhesion and mineralization. Acta Biomater. 7(2):568-77. Assay: ALP in Mouse Preosteoblast cells grown on nanocomposites film (Pubmed).

12. Kauther, MD et al (2010). Alpha-calcitonin gene-related peptide can reverse the catabolic influence of UHMWPE particles on RANKL expression in primary human osteoblasts. Int J Biol Sci.6(6):525-36. Assay: ALP in Human Primary osteoblasts, lysate (Pubmed).

13. Hutchins, HL et al (2011). Eicosapentaenoic acid decreases expression of anandamide synthesis enzyme and cannabinoid receptor 2 in osteoblast-like cells. J Nutr Biochem. 22(2):195-200. Assay: ALP in Mouse osteoblast cells (Pubmed).

14. Pali?, D et al (2011). Use of rapid cytochemical staining to characterize fish blood granulocytes in species of special concern and determine potential for function testing. Fish Shellfish Immunol. 30(2):646-52. Assay: ALP in Fish Granulocytes (Pubmed).

15. Katuru, R et al (2011). Mevalonate depletion mediates the suppressive impact of geranylgeraniol on murine B16 melanoma cells. Exp Biol Med (Maywood) 236(5):604-13. Assay: ALP in Mouse B16 cell lysate (Pubmed).

16. Stewart, AA et al (2008). Effect of dexamethasone supplementation on chondrogenesis of equine mesenchymal stem cells. Am J Vet Res. 69(8):1013-21. Assay: ALP in Horse Mesenchymal stem cells (Pubmed).

17. Milne, TJ et al (2009). Induction of osteopenia during experimental tooth movement in the rat: alveolar bone remodelling and the mechanostat theory. Eur J Orthod. 31(3):221-31. Assay: ALP in Rat serum (Pubmed).

18. Ponnapakkam, T et al (2010). A treatment trial of vitamin D supplementation in breast-fed infants: universal supplementation is not necessary for rickets prevention in Southern Louisiana. Clin Pediatr (Phila). 49(11):1053-60. Assay: ALP in Human blood (Pubmed).

19. Ku?ukali?-Selimovi?, E et al (2011). Evaluation of bone remodelling parameters after one year treatment with alendronate in postmenopausal women with osteoporosis. Bosn J Basic Med Sci. 11(1):41-5. Assay: ALP in Human serum (Pubmed).

20. Zhi, L et al (2011). Synergistic effect of recombinant human bone morphogenic protein-7 and osteogenic differentiation medium on human bone-marrow-derived mesenchymal stem cells in vitro. Int Orthop. 2011 Apr 13. Assay: ALP in Stem cells culture (Pubmed).

21. Fasina, YO, Thanissery, RR (2011). Comparative efficacy of a yeast product and bacitracin methylene disalicylate in enhancing early growth and intestinal maturation in broiler chicks from breeder hens of different ages. Poult Sci. 90(5):1067-73. Assay: ALP in Chicken Small intestine tissue (Pubmed).

22. Ahmed I.M. Abu Obid Alla. Ph.D. Thesis. Effects of Strontium on Human Osteoblasts Extracted from a Cleidocranial Dysplasia Patient – In Vitro Study. Unviersity of Regensburg 2010. Assay: ALP in Human Cells (Pubmed).

23. Maskiewicz, R (2009). SUBLIMABLE SUSTAINED RELEASE DELIVERY SYSTEM AND METHOD OF MAKING SAME. US Patent application number: 20090220602. Assay: ALP in Enzyme Alkaline phosphatase (Pubmed).

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1. Wan, Y et al (2007). PPAR-g regulates osteoclastogenesis in mice. Nature Med. 13(12): 1496-1503. Assay: ALP in Mouse Serum (Pubmed).

2. Sekiya, S, Suzuki, A (2011). Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors. Nature 475(7356):390-3. Assay: ALP in Mouse Plasma (Pubmed).

3. Kim, HJ et al (2006). Glucocorticoids suppress bone formation via the osteoclast. J Clin Invest. 116(8):2152-60. Assay: ALP in Mouse Serum (Pubmed).

4. Panizo, S et al (2009). RANKL increases vascular smooth muscle cell calcification through a RANK-BMP4-dependent pathway. Circ Res. 104(9):1041-8. Assay: ALP in Rat Vascular smooth muscle cells, lysate (Pubmed).

5. Wang, Y et al (2011). Small interfering RNA knocks down the molecular target of alendronate, farnesyl pyrophosphate synthase, in osteoclast and osteoblast cultures. Mol Pharm. 8(4):1016-24. Assay: ALP in Mouse primary osteoblast cells (Pubmed).

6. Bhattacharya, A et al (2006). Effect of fish oil on bone mineral density in aging C57BL/6 female mice. J. Nutr. Biochem 18(6):372-379. Assay: ALP in Mouse serum (Pubmed).

7. Lian, Q et al (2006). Establishing clonal cell lines with endothelial-like potential from CD9(hi), SSEA-1(-) cells in embryonic stem cell-derived embryoid bodies. PLoS One 1:e6. Assay: ALP in Mouse Stem cell line (Pubmed).

8. Henderson, JA et al (2008). Concurrent differentiation of marrow stromal cells to osteogenic and vasculogenic lineages. Macromol Biosci. 8(6):499-507. Assay: ALP in Rat bone marrow stromal cells (Pubmed).

9. Keskar, V et al (2009). In vitro evaluation of macroporous hydrogels to facilitate stem cell infiltration, growth, and mineralization. Tissue Eng Part A. 15(7):1695-707. Assay: ALP in Human Mesenchymal stem cells grown on hydrogels (Pubmed).

10. Oldinski, RA et al (2010). Synthesis and characterization of a Hyaluronan-polyethylene copolymer for biomedical applications. J Biomed Mater Res B Appl Biomater. 94(2):441-6. Assay: ALP in Human Cells (Pubmed).

11. Gaharwar, AK et al (2011). Assessment of using laponite cross-linked poly(ethylene oxide) for controlled cell adhesion and mineralization. Acta Biomater. 7(2):568-77. Assay: ALP in Mouse Preosteoblast cells grown on nanocomposites film (Pubmed).

12. Kauther, MD et al (2010). Alpha-calcitonin gene-related peptide can reverse the catabolic influence of UHMWPE particles on RANKL expression in primary human osteoblasts. Int J Biol Sci.6(6):525-36. Assay: ALP in Human Primary osteoblasts, lysate (Pubmed).

13. Hutchins, HL et al (2011). Eicosapentaenoic acid decreases expression of anandamide synthesis enzyme and cannabinoid receptor 2 in osteoblast-like cells. J Nutr Biochem. 22(2):195-200. Assay: ALP in Mouse osteoblast cells (Pubmed).

14. Pali?, D et al (2011). Use of rapid cytochemical staining to characterize fish blood granulocytes in species of special concern and determine potential for function testing. Fish Shellfish Immunol. 30(2):646-52. Assay: ALP in Fish Granulocytes (Pubmed).

15. Katuru, R et al (2011). Mevalonate depletion mediates the suppressive impact of geranylgeraniol on murine B16 melanoma cells. Exp Biol Med (Maywood) 236(5):604-13. Assay: ALP in Mouse B16 cell lysate (Pubmed).

16. Stewart, AA et al (2008). Effect of dexamethasone supplementation on chondrogenesis of equine mesenchymal stem cells. Am J Vet Res. 69(8):1013-21. Assay: ALP in Horse Mesenchymal stem cells (Pubmed).

17. Milne, TJ et al (2009). Induction of osteopenia during experimental tooth movement in the rat: alveolar bone remodelling and the mechanostat theory. Eur J Orthod. 31(3):221-31. Assay: ALP in Rat serum (Pubmed).

18. Ponnapakkam, T et al (2010). A treatment trial of vitamin D supplementation in breast-fed infants: universal supplementation is not necessary for rickets prevention in Southern Louisiana. Clin Pediatr (Phila). 49(11):1053-60. Assay: ALP in Human blood (Pubmed).

19. Ku?ukali?-Selimovi?, E et al (2011). Evaluation of bone remodelling parameters after one year treatment with alendronate in postmenopausal women with osteoporosis. Bosn J Basic Med Sci. 11(1):41-5. Assay: ALP in Human serum (Pubmed).

20. Zhi, L et al (2011). Synergistic effect of recombinant human bone morphogenic protein-7 and osteogenic differentiation medium on human bone-marrow-derived mesenchymal stem cells in vitro. Int Orthop. 2011 Apr 13. Assay: ALP in Stem cells culture (Pubmed).

21. Fasina, YO, Thanissery, RR (2011). Comparative efficacy of a yeast product and bacitracin methylene disalicylate in enhancing early growth and intestinal maturation in broiler chicks from breeder hens of different ages. Poult Sci. 90(5):1067-73. Assay: ALP in Chicken Small intestine tissue (Pubmed).

22. Ahmed I.M. Abu Obid Alla. Ph.D. Thesis. Effects of Strontium on Human Osteoblasts Extracted from a Cleidocranial Dysplasia Patient – In Vitro Study. Unviersity of Regensburg 2010. Assay: ALP in Human Cells (Pubmed).

23. Maskiewicz, R (2009). SUBLIMABLE SUSTAINED RELEASE DELIVERY SYSTEM AND METHOD OF MAKING SAME. US Patent application number: 20090220602. Assay: ALP in Enzyme Alkaline phosphatase (Pubmed).

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1. Wan, Y et al (2007). PPAR-g regulates osteoclastogenesis in mice. Nature Med. 13(12): 1496-1503. Assay: ALP in Mouse Serum (Pubmed).

2. Sekiya, S, Suzuki, A (2011). Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors. Nature 475(7356):390-3. Assay: ALP in Mouse Plasma (Pubmed).

3. Kim, HJ et al (2006). Glucocorticoids suppress bone formation via the osteoclast. J Clin Invest. 116(8):2152-60. Assay: ALP in Mouse Serum (Pubmed).

4. Panizo, S et al (2009). RANKL increases vascular smooth muscle cell calcification through a RANK-BMP4-dependent pathway. Circ Res. 104(9):1041-8. Assay: ALP in Rat Vascular smooth muscle cells, lysate (Pubmed).

5. Wang, Y et al (2011). Small interfering RNA knocks down the molecular target of alendronate, farnesyl pyrophosphate synthase, in osteoclast and osteoblast cultures. Mol Pharm. 8(4):1016-24. Assay: ALP in Mouse primary osteoblast cells (Pubmed).

6. Bhattacharya, A et al (2006). Effect of fish oil on bone mineral density in aging C57BL/6 female mice. J. Nutr. Biochem 18(6):372-379. Assay: ALP in Mouse serum (Pubmed).

7. Lian, Q et al (2006). Establishing clonal cell lines with endothelial-like potential from CD9(hi), SSEA-1(-) cells in embryonic stem cell-derived embryoid bodies. PLoS One 1:e6. Assay: ALP in Mouse Stem cell line (Pubmed).

8. Henderson, JA et al (2008). Concurrent differentiation of marrow stromal cells to osteogenic and vasculogenic lineages. Macromol Biosci. 8(6):499-507. Assay: ALP in Rat bone marrow stromal cells (Pubmed).

9. Keskar, V et al (2009). In vitro evaluation of macroporous hydrogels to facilitate stem cell infiltration, growth, and mineralization. Tissue Eng Part A. 15(7):1695-707. Assay: ALP in Human Mesenchymal stem cells grown on hydrogels (Pubmed).

10. Oldinski, RA et al (2010). Synthesis and characterization of a Hyaluronan-polyethylene copolymer for biomedical applications. J Biomed Mater Res B Appl Biomater. 94(2):441-6. Assay: ALP in Human Cells (Pubmed).

11. Gaharwar, AK et al (2011). Assessment of using laponite cross-linked poly(ethylene oxide) for controlled cell adhesion and mineralization. Acta Biomater. 7(2):568-77. Assay: ALP in Mouse Preosteoblast cells grown on nanocomposites film (Pubmed).

12. Kauther, MD et al (2010). Alpha-calcitonin gene-related peptide can reverse the catabolic influence of UHMWPE particles on RANKL expression in primary human osteoblasts. Int J Biol Sci.6(6):525-36. Assay: ALP in Human Primary osteoblasts, lysate (Pubmed).

13. Hutchins, HL et al (2011). Eicosapentaenoic acid decreases expression of anandamide synthesis enzyme and cannabinoid receptor 2 in osteoblast-like cells. J Nutr Biochem. 22(2):195-200. Assay: ALP in Mouse osteoblast cells (Pubmed).

14. Pali?, D et al (2011). Use of rapid cytochemical staining to characterize fish blood granulocytes in species of special concern and determine potential for function testing. Fish Shellfish Immunol. 30(2):646-52. Assay: ALP in Fish Granulocytes (Pubmed).

15. Katuru, R et al (2011). Mevalonate depletion mediates the suppressive impact of geranylgeraniol on murine B16 melanoma cells. Exp Biol Med (Maywood) 236(5):604-13. Assay: ALP in Mouse B16 cell lysate (Pubmed).

16. Stewart, AA et al (2008). Effect of dexamethasone supplementation on chondrogenesis of equine mesenchymal stem cells. Am J Vet Res. 69(8):1013-21. Assay: ALP in Horse Mesenchymal stem cells (Pubmed).

17. Milne, TJ et al (2009). Induction of osteopenia during experimental tooth movement in the rat: alveolar bone remodelling and the mechanostat theory. Eur J Orthod. 31(3):221-31. Assay: ALP in Rat serum (Pubmed).

18. Ponnapakkam, T et al (2010). A treatment trial of vitamin D supplementation in breast-fed infants: universal supplementation is not necessary for rickets prevention in Southern Louisiana. Clin Pediatr (Phila). 49(11):1053-60. Assay: ALP in Human blood (Pubmed).

19. Ku?ukali?-Selimovi?, E et al (2011). Evaluation of bone remodelling parameters after one year treatment with alendronate in postmenopausal women with osteoporosis. Bosn J Basic Med Sci. 11(1):41-5. Assay: ALP in Human serum (Pubmed).

20. Zhi, L et al (2011). Synergistic effect of recombinant human bone morphogenic protein-7 and osteogenic differentiation medium on human bone-marrow-derived mesenchymal stem cells in vitro. Int Orthop. 2011 Apr 13. Assay: ALP in Stem cells culture (Pubmed).

21. Fasina, YO, Thanissery, RR (2011). Comparative efficacy of a yeast product and bacitracin methylene disalicylate in enhancing early growth and intestinal maturation in broiler chicks from breeder hens of different ages. Poult Sci. 90(5):1067-73. Assay: ALP in Chicken Small intestine tissue (Pubmed).

22. Ahmed I.M. Abu Obid Alla. Ph.D. Thesis. Effects of Strontium on Human Osteoblasts Extracted from a Cleidocranial Dysplasia Patient – In Vitro Study. Unviersity of Regensburg 2010. Assay: ALP in Human Cells (Pubmed).

23. Maskiewicz, R (2009). SUBLIMABLE SUSTAINED RELEASE DELIVERY SYSTEM AND METHOD OF MAKING SAME. US Patent application number: 20090220602. Assay: ALP in Enzyme Alkaline phosphatase (Pubmed).

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