Nycodenz (R), 60 % (w/v) solution in water

Non-ionic density gradient medium, similar to the former Metrizamide but less toxic. Mammalian cells and viruses isolated in Nycodenz® gradients potentially retain better functional integrity than in Metrizamide gradients. Nycodenz® is suitable for the isolation of a wide range of different cell types, viruses, subcellular organelles and other membrane compartments. Gradient preparation and resolution of cellular organelles are largely similar with both media. In contrast to Metrizamide, solutions of Nycodenz® can be sterilized by autoclaving.