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Neutral Protease NB

For Pancreatic Islet Isolation and Other Dissociation

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Neutral Protease NB is a metalloprotease suitable for tissue dissociation applications. This enzyme cuts diverse peptides in front of hydrophobic amino acids and thereby creates smaller fragments. For cell isolation, Neutral Protease NB is often used in combination with purified Collagenase NB 1 Premium Grade as well as Collagenase NB 4 Standard Grade or Collagenase NB 8 Broad Range. If cells are intended for transplantation into humans, Neutral Protease NB GMP Grade is recommended.

Cat#	Size	Price	Qty	Buy
S3030111	50 U (DMC)	£157.34
S3030112	100 U (DMC)	£225.98

Additional Information

Property	Value or Rating
Manufacturer	Nordmark Biochemicals
Storage	+2 °C to +8 °C
References	References and Definitions Unit definition: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1). References: 1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.

References and Definitions

Unit definition: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).

References:
1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.

Neutral Protease NB

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Neutral Protease NB

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