MICAL-1 Protein 6xHis-tagged

MICAL (MIC01) is an intracellular flavoprotein monoxygenase, conserved from insects to mammals, that functions as a catalyst for oxidation-reduction (redox) reactions. Terman’s group showed that MICAL interacts with F-actin and uses NADPH as a cofactor to oxidize actin at Met44 and Met47. As part of the MOXtrue™ product line, Human MICAL1 protein (MIC01) [accession # Q8TDZ2 (MICA1_HUMAN)] has been produced and purified from a bacterial expression system. The recombinant protein is N-terminally 6x Histidine tagged and is comprised of the Redox and CH domains (aa: 1-612) of the full length protein. Validation studies have shown that MIC01 can oxidize actin which will reduce subtilisin A cleavage by >90% in limited proteolysis assays. MIC01 has also been validated for the production of oxidized actin which can be used in downstream applications such as sedimentation assays and actin polymerization assays.