Metal Chelate Mini Sample Kit - 4 Mini MC Plugs includes buffers

The Proteus IMAC kit is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions. Proteus spin columns replace lengthy and expensive chromatographic methods such as FPLC. Metal chelate affinity chromatography is a rapid one-step purification, which removes most contaminants and can achieve purities close to homogeneity. Contents: Quantity: 4 x 0.23 ml Ni-IMAC columns Max. sample volume per load: 0.65 ml, fixed angle rotor Collection tube: 2.2 ml microfuge tubes Min. number of purifications: 8 purifications (2 uses per column) Typical capacity/preparation: 1 mg 6 x His-tagged protein Vivaspin 500 ultrafiltration concentrators: 4 Buffer: 5 x PBS Buffer A, 1 x Imidazole Buffer B