EnzyLight™ ATP Assay KitEnzyLight™ ATP Assay Kit
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EnzyLight™ ATP Assay Kit

For rapid, quantitative, bioluminescent determination of ATP and evaluation of drug effects on ATP metabolism.

• Safe. Non-radioactive assay. 

• Sensitive and accurate. As low as 0.02 μM ATP or a single cell can be quantified.

• Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.

• Robust and amenable to HTS: Z’ factors of > 0.5 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

Adenosine 5’-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as “energy currency" of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery. BioAssay Systems’ EnzyLight™ ATP Assay Kit provides a rapid method to measure intracellular ATP. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.

Cat# Size Price Qty Buy
EATP-100 100 Tests £254.15

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For rapid, quantitative, bioluminescent determination of ATP and evaluation of drug effects on ATP metabolism.
Method Luminescence
Samples Cells etc
Species All
Detection Limit 0.1 μM
Storage -20 °C
Shelf Life 12 months

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