EnzyFluo™ NADP/NADPH Assay KitEnzyFluo™ NADP/NADPH Assay Kit
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EnzyFluo™ NADP/NADPH Assay Kit

For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.

• Sensitive and accurate. Detection limit of 0.01 μM and linearity up to 1 μM NADP+/NADPH in 96-well plate assay.

• Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 min. Room temperature assay.

• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable. BioAssay Systems’ EnzyFluo™ NADP+/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and with minimal interference (<1%) by NAD+/NADH. Our assay is a convenient method to measure NADP+, NADPH and their ratio.

Cat# Size Price Qty Buy
EFNP-100 100 Tests £343.65

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.
Method FL530/585nm
Samples Cell, tissue extracts etc
Species All
Detection Limit 0.01 μM
Storage -20°C
Shelf Life 6 months

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