EnzyChrom™ Cholesterol Assay KitEnzyChrom™ Cholesterol Assay Kit
Move your mouse over image or click to enlarge

EnzyChrom™ Cholesterol Assay Kit

For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism.

• Sensitive and accurate. Detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay.

• Convenient. Room temperature assay. No 37°C heater is needed. 

• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

CHOLESTEROL is a sterol and lipid present in the cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones, and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; whereas, low levels (hypocholesterolemia) may be linked to depression, cancer and cerebral hemorrhage. Simple, direct and automation-ready procedures for measuring cholesterol are very desirable. BioAssay Systems’ EnzyChrom™ Cholesterol Assay is based on cholesterol esterase hydrolysis of cholesterol esters to form free cholesterol and cholesterol dehydrogenase catalyzed conversion of cholesterol to cholest-4-ene-3-one, in which NAD is reduced to NADH. The optical density of the formed NADH at 340 nm is directly proportionate to the cholesterol concentration in the sample.

Cat# Size Price Qty Buy
ECCH-100 100 Tests £326.65

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism.
Method OD340nm
Samples Serum, plasma etc
Species All
Detection Limit 5 mg/dL
Storage -20°C
Shelf Life 6 months
References Assay: Cholesterol in Rat serum (Pubmed).

2. Rubinstein J et al (2009). Hypercholesterolemia and myocardial function evaluated via tissue doppler imaging. Cardiovasc Ultrasound. 7:56. Assay: Cholesterol in Rabbit serum (Pubmed).

3. Hu, M et al. Effect of prolonged starvation on body weight and blood-chemistry in two horseshoe crab species: Tachypleus tridentatus and Carcinoscorpius rotundicauda (Chelicerata: Xiphosura). Assay: Cholesterol in Horseshoe crab Plasma (Pubmed).

" title="

1. Henein, HY et al (2011). Effect of adrenomedullin gene delivery on insulin resistance in type 2 diabetic rats. J Adv Res 2(1): 57-64 . Assay: Cholesterol in Rat serum (Pubmed).

2. Rubinstein J et al (2009). Hypercholesterolemia and myocardial function evaluated via tissue doppler imaging. Cardiovasc Ultrasound. 7:56. Assay: Cholesterol in Rabbit serum (Pubmed).

3. Hu, M et al. Effect of prolonged starvation on body weight and blood-chemistry in two horseshoe crab species: Tachypleus tridentatus and Carcinoscorpius rotundicauda (Chelicerata: Xiphosura). Assay: Cholesterol in Horseshoe crab Plasma (Pubmed).

" target="_blank">

1. Henein, HY et al (2011). Effect of adrenomedullin gene delivery on insulin resistance in type 2 diabetic rats. J Adv Res 2(1): 57-64 . Assay: Cholesterol in Rat serum (Pubmed).

2. Rubinstein J et al (2009). Hypercholesterolemia and myocardial function evaluated via tissue doppler imaging. Cardiovasc Ultrasound. 7:56. Assay: Cholesterol in Rabbit serum (Pubmed).

3. Hu, M et al. Effect of prolonged starvation on body weight and blood-chemistry in two horseshoe crab species: Tachypleus tridentatus and Carcinoscorpius rotundicauda (Chelicerata: Xiphosura). Assay: Cholesterol in Horseshoe crab Plasma (Pubmed).

Related Documents