EnzyChrom™ Alanine Transaminase Assay KitEnzyChrom™ Alanine Transaminase Assay Kit
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EnzyChrom™ Alanine Transaminase Assay Kit

For quantitative determination of alanine transaminase activity.

• Sensitive. Linear detection range: 2-100 U/L. 

• Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min.

Alanine Transaminase (ALT), also known as serum alanine aminotransferase (ALAT) or pyruvic transaminase (SGPT), facilitates the conversion of alanine and a-ketoglutarate to pyruvate and glutamate. ALT plays an important role in gluconeogenesis and amino acid metabolism. ALT is found mainly in the liver, and, to a lesser extent, in kidney, heart, muscle, and pancreas tissues. Normal serum levels of ALT are low, and increased serum ALT activity is widely used as a marker for liver damage. Simple, direct and automation-ready procedures for measuring ALT activity find wide applications in research and drug discovery. BioAssay Systems’ ALT activity assay is based on the quantification of pyruvate produced by ALT. In this assay, pyruvate and NADH are converted to lactate and NAD by the enzyme lactate dehydrogenase (LDH). The decrease in NADH absorbance at 340 nm is proportional to ALT activity.

Cat# Size Price Qty Buy
EALT-100 100 Tests £267.15

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of alanine transaminase activity.
Method OD340nm
Samples Serum, plasma etc
Species All
Detection Limit 2 U/L
Storage -20°C
Shelf Life 6 months
References Assay: Alanine Transaminase in mice serum (Pubmed).

2. Rattan R et al (2011). Metformin suppresses ovarian cancer growth and metastasis with enhancement of cisplatin cytotoxicity in vivo. Neoplasia 13(5):483-491. Assay: Alanine Transaminase in Mouse Plasma (Pubmed).

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1. Mani V et al (2012) Effects of the Total Alkaloidal Extract of Murraya koenigii Leaf on Oxidative Stress and Cholinergic Transmission in Aged Mice. Phytotherapy Research . Assay: Alanine Transaminase in mice serum (Pubmed).

2. Rattan R et al (2011). Metformin suppresses ovarian cancer growth and metastasis with enhancement of cisplatin cytotoxicity in vivo. Neoplasia 13(5):483-491. Assay: Alanine Transaminase in Mouse Plasma (Pubmed).

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1. Mani V et al (2012) Effects of the Total Alkaloidal Extract of Murraya koenigii Leaf on Oxidative Stress and Cholinergic Transmission in Aged Mice. Phytotherapy Research . Assay: Alanine Transaminase in mice serum (Pubmed).

2. Rattan R et al (2011). Metformin suppresses ovarian cancer growth and metastasis with enhancement of cisplatin cytotoxicity in vivo. Neoplasia 13(5):483-491. Assay: Alanine Transaminase in Mouse Plasma (Pubmed).

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