EnzyChrom™ Acetate Assay KitEnzyChrom™ Acetate Assay Kit
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EnzyChrom™ Acetate Assay Kit

For quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism.

• Use as little as 10 μL samples. Detection range: 0.20 to 20 mM acetate for colorimetric assays and 0.13 to 2 mM for fluorimetric assays.

Acetate is a common anion and fundamental to all forms of life. When bound to coenzyme A, it is central to the metabolism of carbohydrates and fats. It is acid form, acetic acid, is produced and excreted by acetic acid bacteria, such as Acetobacter genus and Clostridium acetobutylicum, which are found universally in foodstuffs, water, and soil. Acetic acid is also a component of the vaginal lubrication of humans and other primates, where it appears to serve as a mild antibacterial agent. Acetic acid is the main component of vinegar, and extensively used in food, dyes, paints, glue and synthetic fibres. BioAssay Systems’ assay uses enzyme-coupled reactions to form a colored, fluorescent product. The color absorbance at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the acetate concentration in the sample.

Cat# Size Price Qty Buy
EOAC-100 100 Tests £318.15

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism.
Method OD570nm, or FL530/585nm
Samples Serum, plasma, food, agriculture and environment 
Species All
Detection Limit 0.13 mM
Storage -20°C
Shelf Life 6 months
References Assay: Acetate in Microbe cell (Pubmed).

2. Cuervo-Soto, LI et al (2015). Identification of a novel carbohydrate esterase from Bjerkandera adusta: Structural and function predictions through bioinformatics analysis and molecular modeling. Proteins: Structure, Function, and Bioinformatics 83(3): 533-546. Assay: Acetate in Microbe culture (Pubmed).

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1. Garrido D et al (2013). Utilization of galactooligosaccharides by Bifidobacterium longum subsp. infantis isolates. Food Microbiol. 33(2):262-70. Assay: Acetate in Microbe cell (Pubmed).

2. Cuervo-Soto, LI et al (2015). Identification of a novel carbohydrate esterase from Bjerkandera adusta: Structural and function predictions through bioinformatics analysis and molecular modeling. Proteins: Structure, Function, and Bioinformatics 83(3): 533-546. Assay: Acetate in Microbe culture (Pubmed).

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1. Garrido D et al (2013). Utilization of galactooligosaccharides by Bifidobacterium longum subsp. infantis isolates. Food Microbiol. 33(2):262-70. Assay: Acetate in Microbe cell (Pubmed).

2. Cuervo-Soto, LI et al (2015). Identification of a novel carbohydrate esterase from Bjerkandera adusta: Structural and function predictions through bioinformatics analysis and molecular modeling. Proteins: Structure, Function, and Bioinformatics 83(3): 533-546. Assay: Acetate in Microbe culture (Pubmed).

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