Product Uses Include
- Study of GEF activity with different GTPases.
- Identification of Dbs binding proteins
- Study of Dbs function in vivo by the introduction of His-Dbs into live cells
- Postive control for other RhoGEF studies.
Material
The Guanine nucleotide Exchange Factor domain (GEF domain, also known as the DH/PH tandem domain) of the human RhoGEF Dbs (hDbs) has been produced in a bacterial expression system with a 6xHis tag at its amino terminus. Dbs is an efficient GEF for the RhoA and Cdc42 GTPases and it shows weak GEF activity towards Rac1.
The molecular weight of this His-hDbs DH/PH protein is approximately 40 kDa. The protein is supplied as a lyophilized powder. When reconstituted to 2 mg/ml, the protein is in the following buffer: 20 mM Tris pH 7.5, 0.5 mM MgCl2, 0.5% sucrose and 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent (Cat. # ADV02).
Purity
Purity is determined by scanning densitometry of proteins run on SDS-PAGE gels. Samples are >80% pure.
Biological Activity
The biological activity of the hDbs DH/PH was tested by measuring the ablility of the protein to catalyze the exchange of GDP for mant-GTP on Cdc42 and RhoA (Cat. # CD01 and RH01, respectively) using the RhoGEF exchange assay kit (Cat. # BK100).
