Collagenase AF-1 GMP GradeCollagenase AF-1 GMP Grade
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Collagenase AF-1 GMP Grade

For Cell Isolation from Pancreas, Adipose Tissue and Tumors

Collagenase AF-1 GMP Grade is derived from C.histolyticum using a production process completely free of any animal-based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This highly purified collagenase, often used in combination with Neutral Protease AF GMP Grade, is suitable for the dissociation of sensitive cells from several tissues or organs such as pancreas, liver or tumors. It has been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation, stromal vascular fraction (SVF) from adipose tissue and tumor cells.
Cat# Size Price Qty Buy
N0003554 ≥ 2,000 U (PZ) £1,773.02

Additional Information

Property Value or Rating
Product Size ≥ 2,000 U (PZ)
Manufacturer Nordmark Biochemicals
Storage +2 °C to +8 °C
References

References and Definitions

*Unit definitions: Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
**Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO appoved reference standard endotoxin (RSE)].

References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51

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