The BlastR™ rapid lysate prep kit allows for fast, reliable, and quantitative preparation of genomic DNA (gDNA)-free cell lysate for western blot or immunoprecipitation. The benefit of this prep kit is a combination of the unique denaturing BlastR™ buffer combined with the proprietary BlastR™ filters. The denaturing BlastR™ buffer allows for complete protein isolation from all cellular compartments, but its composition allows for easy protein quantiation with conventional protein assays unlike most denaturing buffers such as Laemmli. Additionally, the BlastR™ filter effectively removes gDNA contamination which is a significant problem with denaturing buffers that can affect downstream applications like immunoprecipitation and migration in SDS-PAGE. Simply put, the BlastR™ rapid lysate prep kit provides all the superior benefits of using a denaturing buffer system with the ease of performing a standard non-denaturing lysis buffer preparation. The BlastR™ rapid lysate prep kit is an integral component of our Signal-Seeker™ products and is efficient for studying protein modifications for any target protein.
Product Uses Include
- Isolate total protein profile from all cellular organelles
- Reduce viscosity of samples
- Rapid isolation of protein extracts
- Effective buffer system for studying an array of PTMs
Sample Kit Components (50 filters and sample preps)
- BlastR™ Filter set
- BlastR™ Lysis buffer
- BlastR™ Dilution buffer
- 5X Laemmli buffer
- Blanking buffer
- Precision Red Advanced Protein Assay Reagent
