Tris-(2-carboxyethyl)phosphine hydrochloride

Water-soluble and odorless reagent for selective and fast reduction of disulfides. Does not react with other functional groups of proteins. Unreactive towards many common alkylating reagents, so reductions have been carried out simultaneously with alkylations. More stable and effective than DTT. The strength of the phosphorus-oxygen bond makes the reaction irreversible. Dilute solutions of TCEP (1 mM) react rapidly at room temperature. Suitable also in mass spectrometry applications.