Tau protein - bovine brain
Tau protein - bovine brain (TA01-GRP)
Products
Description
Product Uses Include
- Positive control for the study of microtubule binding proteins
- Investigation of the the effect of Tau on microtubule dynamics
Material
Tau protein is isolated from MAP-rich bovine brain tubulin by ion exchange chromatography over a phosphocellulose matrix. The protein is supplied lyophilized. When reconstituted to 1 mg/ml (protein concentration determined by Precision Red Advanced Protein Assay, Cat. # ADV02), the protein will be in in 50 mM PIPES, 1 mM EDTA and 16 mM NaCl.
Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are approximately 90% Tau proteins. Approximatley 1% contamination by high molecular weight MAPs and 9% other MAPs and non-MAP proteins.
Biological Activity
Biological activity of is determined by the ability of Tau protein to enhance the polymerization rate (Vmax) of purified bovine brain tubulin (Cat. # TL238) in vitro. Stringent quality control ensures that the Tau protein will stimulate tubulin polymerization approximately 20-fold when compared to tubulin polymerization without Tau fraction.
Specifications
| Storage | 4°C |
|---|