SIR650-BG (CY-SC504)

SiR650-BG is the benzylguanine derivative of our SiR fluorophore. It emits light in the far-red, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SiR650-BG can be imaged with a standard Cy5 filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as SNAP-Cell® 647-SiR and also identical to SiR-SNAP, the name used in its original publication in Nature Chemistry1). Benzylguanine (BG) is the substrate of the self labeling tag SNAP-tag™*. Upon reaction with a BG derivative, SNAP-tag™* forms a covalent bond with the substrate and releases guanine. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as SNAP-tag™* fusion.