SiR-Alkyne (SC010-GRP)

SiR-alkyne is the N-propargylcarboxamide derivative of the fluorophore silicon rhodamine (SiR). Sir-alkyne reacts with azide derivatives in the presence of copper (I) to form 1,2,3-triazoles linkage. Small molecules, peptides, oligonucleotides or proteins bearing the appropriate azide can be conjugated to SiR-alkyne. SiR excitation and emission are compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with a standard Cy5 filterset.

Properties

λ_abs 652 nm
ε_max 1.0·10⁵ mol^-1·cm^-1
λ_fl 674 nm
MW =509.7 g/mol
CF₂₆₀ = 0.116
CF₂₈₀ = 0.147