SERVALight PreMix Helios CL HRP WB Substrate (42657.01)

SERVALight PreMix chemiluminescent horseradish peroxidase (HRP) substrates are ready-to-use solutions for convenient and fast detection of proteins in Western Blotting. The pre-mixed solutions save time and increase consistency in your results, avoiding pipetting errors and possible contaminations. SERVALight PreMix Helios is an extremely sensitive HRP Western Blot substrate with excellent signal intensity, which enables to detect very low amounts of proteins reducing the needed quantitiy of antibodies. 0.1 ml substrate is sufficient for one cm2 membrane. Extreme sensitivity, low femtogram limit of detection No mixing, ready-to-use at room temperature Low background, high signal/noise ratio Primary antibody dilution 1:5000 -1:100,000 Secondary antibody dilution 1:100,000 – 1:500,000 Signal duration 2 hours